This volume explores the latest techniques used to study how mesoscale imaging can be applied in various areas of neuroscientific research. The chapters in this book are organized into four parts: Part One highlights novel imaging modalities, such as neurotransmitter and single-cell resolution imaging and analyses of neuroimaging data in awake rodents. Part Two covers technical innovations such as head-mounted camera systems, multi-site photometry, and concurrent imaging-electrographic probes. Part Three presents methods of mesoscale imaging during normal behavior, and Part Four looks at the mesoscale imaging methods used to study neurological diseases and disorders that are critical to observe in the non-anesthetized condition. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and thorough, Awake Behaving Mesoscopic Brain Imaging is a valuable resource for both novice and expert researchers who are interested in learning more about this exciting and innovative topic.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains keynotes and implementation advice from the experts

Inhalt

Mesoscopic Imaging of Neurotransmitters and Neuromodulators with Genetically Encoded Sensors.- Analysis of Mesoscope Imaging Data.- Real-Time Ultra-Large-Scale Imaging with High-Resolution Microscopy.- Mesoscale Calcium (Ca2+) Imaging in Freely Behaving Mice.- High-Density Multi-Channel Fiber Photometry.- Widefield Imaging Combined with a Transparent Electrographic Probe.- Wide-Field Calcium Imaging of Mesoscale Networks Underlying the Encoding of Skilled Voluntary Movement.- Technical Considerations for Widefield Optical Imaging during Visuomotor Behaviors.- Mesoscale Ca2+ Imaging during Locomotion.- Mesoscale Ca++ Imaging of Seizures and Interictal Activity in Models of Chronic Epilepsy.- Mesoscale Imaging of Neural Dynamics in Epilepsy.- Mesoscale Imaging of Stroke.