This volume looks at the latest methodological developments used in the field to investigate the synaptic organization of the brain from different viewpoints including structural, structural/functional, molecular, and computational methods. The chapters in this book are organized into five parts and cover topics such as preparation of brain tissue and techniques; connectivity; synaptic vesicles; phenomenological modeling; and dendritic spines. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Authoritative and thorough, New Aspects in Analyzing the Synaptic Organization of the Brain is a valuable resource that will help students, post-docs, and even expert researchers learn more about the discipline of cellular and synaptic neuroscience.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Long-Distance Transport of Surgically Resected, Alive Human Brain Tissue.- Modern Glyoxal Fixation for the Determination of the Copy Number of Synaptic Vesicles Proteins at Individual Synapses.- Cell Labeling and FIB-SEM Imaging.- Unbiased Sampling and Volume-Sorting of Origin-Specific Terminals Using SBEM Image Stacks.- A Technique to Perform Structure-Function Analysis of Synapses.- Large Volume Electron Microscopy.- Espina: A Software Tool for the Study of 3D Synaptic Organization.- Automated Imaging and Analysis of Synapses in Freeze-Fracture Replica Samples with Deep Learning.- Multiplexed d STORM Imaging of Neuronal Tissue and Cells.- Detailed Phenotyping of the Physiology, Structure, and Connectivity of Individual Neocortical Neurons.- 3D Quantitative Analysis of Synaptic Ultrastructure in Identified Long-Range Projection Axons.- Quantitative Analysis of the Synaptic Organization of the Human Temporal Lobe Neocortex.- Counting Synaptic Vesicles: As Simple as That?.- Readiy Releasable Pool.- Phenomenological Modeling of Diverse and Heterogeneous Synaptic Dynamics at Natural Density.- Computational Methods and Algorithms to Segment and Model Recurrent Bursting Events in Long-Time Series.- Probing Dendritic Function with Holographic Structured Illumination.- Intravital Imaging and Analysis of the Structural Plasticity of Dendritic Spines in Multiple Brain Regions.