This volume looks at the latest advancements at the forefront of cellular, circuit, and neural network imaging in the brain. The chapters in this book cover topics such as super resolution imaging at the level of molecules and nanoscopic cellular compartments; the monitoring of individual synapses; physiological communication inside and among neurons and astrocytes; and the activity of targeted multi-cellular ensembles and selected populations of neurons. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Fluorescence Imaging of the Brain is a valuable resource for both novice and experienced researchers who are interested in learning more about this rapidly evolving area of optical methods and approaches that employ fluorescence sensors to better understand the versatile brain machinery.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

The Y-Maze Test Combined with Fluorescence Recordings for Assessing Neural Correlates of Cognition.- Quantitative Optical Analysis of Astrocytic Ca2+ Signaling.- Neuron Segmentation from Noisy Fluorescence Microscopy Images using Deep Learning with Local and Global Scale Fusion.- In Vivo Calcium and Neurotransmitter Fluorescence Imaging in Acute Models of Epilepsy.- Synaptic Photodamage and Its Application to Study Microglia-Synapse Interactions In Vivo.- Virtually Induced CRISPR/Cas9-Based Knock-In of Fluorescent Albumin Allows Long-Term Visualization of Cerebral Circulation in Infant and Adult Mice.- Multiplexed Imaging of Glutamate Release and Local Intracellular Ca2+ Dynamics with the Halotag System.- Mapping Neuron-Astrocyte Circuits with CaMPARIGFAP.- Measuring Ca2+ Dynamics in the Astrocytic Syncytium with FLIM: Side-by-Side Comparison of Blous- vs Patch-Loading of Ca2+ Sensitive Dyes.- pHluorins: A Versatile Tool for the Study of Vesicle Biology.- Identification of State-Dependent Encoding of Innate Rewards by Deep-Brain Single-Cell Imaging in Freely Behaving Mice.- Targeted AAV Transduction for Fluorescent Imaging of the Tripartite Synapse.- A STED Microscope for Multimodal Investigation of Dendritic Spine Structure and Function.- Investigating Presynaptic Activity using the SF-iGluSnFR Sensor: Measuring Kinetics and Plasticity of Glutamate Release Across Heterogenous Population of Synapses.- High-Throughput Analysis of Subcellular Ratiometric Imaging Data: Cyclic AMP in Astrocytes.