This volume details techniques involved in the study of endosymbionts. Chapters divided into four sections detail isolation, maintenance of diverse endosymbionts, classical microscopy, staining, PCR, FISH, flow cytometry, next-generation sequencing, endosymbionts, metabolites, and bacterial endosymbionts in insects. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs.

Authoritative and cutting-edge, Endosymbionts aims to empower researchers to expand the frontiers of endosymbiosis research and unlock new possibilities for science.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Isolation and maintenance of endohyphal symbionts from the fungi.- Isolation and maintenance of algal endosymbionts from ciliates.- Isolation and maintenance of methanogenic endosymbionts.- Isolation and maintenance of cyanobacterial endosymbionts.- Isolation of fungal endosymbionts.- Isolation and Characterisations of Endosymbionts from Slime Mold Fruiting Bodies.- Isolation of Endosymbiotic Actinobacteria.- Isolation of Endosymbionts from Green Algae.- Isolation and identification of culturable endosymbiotic bacteria from insects.- Isolation and maintenance of endosymbionts from mycorrhiza.- Isolation and maintenance of endosymbionts from lichens.- Microscopy and staining-based identification of endosymbionts.- PCR based approaches to study endosymbionts.- Non-PCR Based Approaches to Study Endosymbionts.- Single cell-based technologies to study endosymbionts: Raman microspectroscopy.- Next generation sequencing approaches to study endosymbionts.- Screening of endosymbionts for plant growth promoting metabolites.- Screening of endosymbionts for EPS production for enhancement of symbiotic proficiency with the host.- Screening of Endosymbionts for Biocontrol Properties.- Screening of endosymbionts for virucidal activity.- Screening of endosymbionts for bactericidal activity.- Screening of endosymbionts for anticancer compounds.- Screening of Endosymbionts for Nematicidal Activity.- Screening of endosymbionts for biosurfactant production.- Screening of endosymbionts as anthelmintic agent.- Screening of endosymbionts for antibiotic and probiotic activity.- Diagnostic PCR to Detect Infection of Bacterial Endosymbionts in Insects.- Fluorescent in situ hybridization for the detection of bacterial infection of endosymbionts in insects.