This second edition provides new and updated methods focusing on pollen cryopreservation in various crops . Chapters detail specific plant groups, addressing a wide range of speciesfrom cereals and grasses to fruits, nuts, vegetables, ornamentals, and medicinal plants. It features protocols for species such as Semecarpus anacardium , grapes, strawberries, cashew, soybean, amaryllis, and many others, offering practical guidance for researchers and breeders working across diverse agricultural and horticultural domains.Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs.

Authoritative and cutting-edge, Pollen Cryopreservation Protocols, Second Edition aims to serve as reference for researchers studying plant breeding and plant conservation biology.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Enhancing Hybrid Seed Production through Pollen Cryopreservation.- Applications in Biotechnology and Crop Improvement.- Technological Innovations in Pollen Cryopreservation.- Pollen Cryopreservation Protocol for Abelmoschus moschatus subsp. moschatus Medik.- Pollen cryopreservation of Pandanus fascicularis.- Optimized Cryopreservation Protocol for Pteridophyte Spores.- Cryopreservation of Paspalum spp. pollen grains.- Pollen cryopreservation protocol for Rice (Oryza sativa L.).- Pollen Cryopreservation Protocol for Appemidi Mango.- Pollen cryopreservation in Star fruit (Averrhoa carambola L.).- Pollen Cryopreservation in Cashew (Anacardium occidentale L).- Pollen Cryopreservation in Grape Species.- Pollen Cryopreservation of Semecarpus anacardium.- Extracting and Storing Strawberry Pollen.- Pollen Cryopreservation of Agave americana L..- Protocol for the Pollen cryopreservation of Terminalia paniculata B.Heyne ex Roth.- Pollen Cryopreservation in Luffa Species.- Cowpea Pollen Cryopreservation [Vigna Unguiculata (L.) Walp.].- Pollen Cryopreservation in Grasspea (Lathyrus sativus L.).- Pollen cryopreservation protocol for soybeanGlycine maxL..- Pollen Cryopreservation of Elaeis guineensis.- Optimizing Pollen Cryopreservation in Asclepias curassavica.- Pollen Cryopreservation protocol for Barleria obtusa Nees.- Pollen Cryopreservation of Bauhinia purpurea L..- Pollen cryopreservation of Cosmos sulphureus Cav. and Coreopsis lanceolata L.- Pollen cryopreservation in Bird of paradise.- Pollen Cryopreservation of Brownea grandiceps Jacq..- Pollen Cryopreservation of Cassia fistula.- Protocol for the Pollen Cryopreservation of Clitoria ternatea L..-Pollen cryopreservation protocol for Costus species.- Pollen cryopreservation protocol for Indian Crinum spp..- Pollen cryopreservation protocol for Hemerocallis fulva.- Pollen cryopreservation in ornamental Araceae member: Homalomena rubescens (Roxb.) Kunth.- Pollen Cryopreservation of Hydrocera triflora (L.) Wight & Arn..- Pollen cryopreservation protocol for Spider Lilly (Hymenocallis speciosa Salisb.).- Cryopreservation of Pollen Grains of Jatropha spp..- Pollen Cryopreservation of Millingtonia hortensis.- Pollen Cryopreservation Protocol for Indian Waterlilies (Nymphaea spp.).- Pollen Cryopreservation protocol for the genus Nymphoides Ség..- Cryopreservation of the pollinia of selected Orchid Hybrids.- Pollen cryopreservation of Spathiphyllum wallissi Regel.- Pollinia Cryopreservation of Spathoglottis plicata Blume.- Pollen Cryopreservation of Torenia bicolor Dalzell.- Pollen Cryopreservation of Kigelia africana (Lam.) Benth.- Pollen cryopreservation protocol for Acorus calamus.- Pollen Cryopreservation Protocol for Alpinia calcarata.- Pollen cryopreservation in Alpinia galanga.- Pollen cryopreservation in Andrographis paniculata.- Pollen cryopreservation in Bacopa monneri.- Pollen Cryopreservation of Sappan wood.- Cryopreservation protocol for the pollinia of Calotropis gigantea (L.) W.T.Aiton.- Pollen Cryopreservation of Catharanthus roseus (L.) G.Don .- Eclipta alba pollen cryopreservation protocol.- Gymnema sylvestre pollinia cryopreservation.- Pollen cryopreservation in Indian Sarsaparilla (Hemidesmus indicus L.).- Pollen Cryopreservation Protocol for Rauvolfia serpentina.- Cryopreservation of pollinia of Tylophora indica.- Pollen cryopreservation in Ashwagandha (Withania somnifera L.).