This volume provides researchers and students with an introduction to the identification and transmission of plant viruses as well as providing them with practical methodologies that demonstrate the detection of plant viruses through immunological and molecular methods.

Authoritative and cutting-edge, Detection of Plant Viruses: Advanced Techniques aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Introduction.- Identification Of Plant Viruses Based On Symptoms.- Mechanical Inoculation Of Plant Viruses.- Transmission Of Plant Virus By Grafting.- Isolation And Identification Of Virus Strains.- Determination Of Dilution End Point Of Viruses.- Thermal Inactivation Of Plant Viruses.- Bioassays Of Plant Viruses.- Direct Enzyme Linked Immuno Sorbent Assay (Elisa) For Detection Of Plant Viruses.- Direct Antigen Coated (Dac) Enzyme Linked Immuno Sorbent Assay (Elisa) For Detection Of Plant Viruses.- Double Antibody Sandwich Enzyme Linked Immuno Sorbent Assay (Elisa) For Detection Of Plant Viruses.- Electron Microscopy For Plant Viral Detection.- Extraction Of High-Quality Genomic Dna From Plants Using Modified Ctab-Based Method.- Extraction Of High-Quality Genomic Rna Using Modified Trizol Methods.- Extraction Of High-Quality Genomic Rna Using Modified Ctab Method.- Extraction Of High-Quality Genomic RNA Using Modified Kit Method.- Gel Electrophoresis For Visualisation Of Nucleic Acid.- Polyacrylamide Gel Electrophoresis For Visualisation Of Nucleic Acid.- Measurement Of RNA/DNA Concentration Using Spectrophotometer.- Polymerase Chain Reaction (Pcr) For Virus Detection.- Reverse Transcription- Polymerase Chain Reaction (Rt-Pcr) For RNA Viruses.- RT-Nested Pcr For Plant Virus Detection.- Real Time PCR (qPCR) For Plant Virus Detection And Expression Analysis.- MULTIPLEX PCR IN PLANT VIRUS DETECTION.- COLONY PCR In Plant Virology.- APPLICATION OF DIGITAL PCR (dPCR) IN PLANT VIRUS DETECTION.- Immuno-Capture Polymerase Chain Reaction (IC-PCR) In Detection Of Plant Viruses.- REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION (RT-LAMP) FOR PLANT VIRAL DETECTION.- RECOMBINASE POLYMERASE AMPLIFICATION FOR VIRAL DETECTION.- DETECTION OF PLANT VIRUSES BY CRISPR-Cas13a SYSTEM.- DETECTION OF PLANT VIRUSES THROUGH RNA-Seq.- PREPARATION OF SEQUENCING LIBRARY FOR OXFORD NANOPORE MINION SEQUENCING.- REDUCED REPRESENTATION METHYLATION SEQUENCING (RRMS) FOR PLANT VIRUSES.- REDUCED REPRESENTATION BISULFITE SEQUENCING (RRBS) FOR PLANT VIRUSES.- REMOTE SENSING FOR VIRUS DETECTION.- ELECTROCHEMICAL METHODS FOR VIRUS DETECTION.- NEXT-GENERATION FLOW CYTOMETRY METHODS FOR PLANT VIRUS DETECTION.- SURFACE PLASMON RESONANCE FOR VIRUS DETECTION IN PLANTS.- IN VIVO SYNTHESIS AND PURIFICATION OF DOUBLE STRANDED RNA (dsRNA) for RNA INTERFERANCE (RNAi).