This third edition expands upon the previous two editions with new and updated methods presenting comprehensive, step-by-step protocols for recently developed techniques that were not included in the earlier volumes. Chapters feature contributions from the globally leading experts in the field, aiming to equip researchers with the practical tools necessary for successful CRISPR experimentation.

Authoritative and cutting-edge, CRISPR-Cas Methods, Volume 3 aims to be a useful and practical guide for researches to help further their study in this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Targeted base editing in rice using Target-AID.- Accelerated laboratory evolution of conjugative plasmids for CRISPR delivery in bacteria.- A SERS-signalled, CRISPR-Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a single tube-in-tube vessel.- Genetic Engineering of Bacteriophage Using CRISPR-Cas12a.- CRISPR-Cas12a-powered dual-mode biosensor for ultrasensitive and cross-validating detection of pathogenic bacteria.- Intronization of coding sequences for optimization of gene expression.- Sensitive small molecule aptasensing-based on hybridization chain reaction and CRISPR-Cas12a using a portable 3D-printed visualizer.- Highly efficient genome editing in plants with the LbCas12a-RRV variant.- CRISPR-Cas13a-based bacterial detection platform: Sensing pathogen Staphylococcus aureus in food samples.- High efficiency Cas12a mediated A-to-G base editing in rice.- A smartphone-based visual biosensor for CRISPR-Cas powered SARS-CoV-2 diagnostics.- Efficient single-cell cloning of genome-edited cultured human cells.- Ultrasensitive pathogenic bacteria detection by a smartphone-read G-quadruplex-based CRISPR-Cas12a bioassay.- A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR-Cas12a and silver nanoclusters.- CRISPR-Cas12a-based aptasensor for sensitive and selective ATP detection.- A portable, visual and dual-mode biosensor for ultrasensitive and self-validating detection of foodborne pathogenic bacteria based on CRISPR-Cas9 system.- CRISPR-Cas12-based PCV2 viral detection.- Surface-enhanced Raman scattering -based CRISPR-Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods.- CRISPR-Cas in woody perennial plants: methods, efficiency, applications, and challenges to creating commercial varieties with high ecological and economic value.- A Unified Protocol for Genome Editing in Monocot and Dicot Plants Using a Transposon-Associated TnpB System.- CRISPR-Cas12a-Based DNA Detection for Rapid Diagnosis of Wheat Blast Disease.