Patch-Clamp Analysis

Continuing the research of the best-selling first edition, Patch-Clamp Analysis: Advanced Techniques, Second Edition collects three more years of research in the ever-expanding study of the cell membrane. With the latest developments in the traditional patch techniques such as whole-cell and single channel as well as perforated patch, fast drug application, loose patch and macropatch techniques, this authoritative second edition updates the standard techniques while introducing three brand new, cutting-edge technical advances in the field.

Thorough and timely, Patch-Clamp Analysis: Advanced Techniques, Second Edition is an invaluable resource for neuroscientists attempting to discover and understand the long-hidden complexities of the cell.

Provides readily reproducible, step-by-step protocols that ensure experimental success Describes methods combining patch-clamp with molecular biology and imaging Includes new state-of-the-art techniques Includes supplementary material: sn.pub/extras

Klappentext

Continuing the research of the best-selling first edition, Patch-Clamp Analysis: Advanced Techniques, Second Edition collects three more years of research in the ever-expanding study of the cell membrane. With the latest developments in the "traditional" patch techniques such as whole-cell and single channel as well as perforated patch, fast drug application, loose patch and macropatch techniques, this authoritative second edition updates the standard techniques while introducing three brand new, cutting-edge technical advances in the field.

Thorough and timely, Patch-Clamp Analysis: Advanced Techniques, Second Edition is an invaluable resource for neuroscientists attempting to discover and understand the long-hidden complexities of the cell.

Inhalt
Technology of Patch-Clamp Electrodes.- Whole-Cell Patch-Clamp Recordings.- Single-Channel Recording.- Combined Fluorometric and Electrophysiological Recordings.- Combining Uncaging Techniques with Patch-Clamp Recording and Optical Physiology.- Visually Guided Patch-Clamp Recordings in Brain Slices.- In Vivo Patch-Clamp Technique.- Perforated Patch-Clamp Techniques.- Fast Drug Application.- Pipette Internal Perfusion: Methods and Applications.- Loose-Patch-Clamp Method.- Recording Currents from Channels and Transporters in Macropatches.- Structure-Function Analyses of Single Cells by Combining Patch-Clamp Techniques with Reverse Transcription-Polymerase Chain Reaction.- Planar Patch Clamping.- Automated Glass Pipette-Based Patch-Clamp Techniques.