Genotoxicity and DNA Repair: A Practical Approach provides a key reference for determining how to analyze the genotoxic activity of molecules or materials and, at the same time, serves as a useful tool for researchers in the Environmental Mutagenesis and DNA Repair fields. Focused on genotoxicity assays recommended by the OECD guidelines for the testing of chemicals, this volume also covers other useful assays, such as some gene mutation assays, the comet assay in different species and applications, and the SMART assays of Drosophila. For all the assays, the book presents brief theoretical introductions to the topics and updated standard and modified step-by-step protocols to perform them. Special emphasis is placed on the analysis of nanoparticles, including an integrative approach analysis. The DNA Repair section includes several assays that provide information on repair activity in vitro and in vivo, as well as recent applications to study DNA repair in humans, cell cultures, and animal models. As a volume in the Methods in Pharmacology and Toxicology series, the chapters contain the kind of detail and key implementation advice that ensures reproducible results in the lab.

Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study.

Focuses on key assays in genotoxicity and DNA repair Places special emphasis on the analysis of nanoparticles and nanomaterials Features reproducible techniques and expert implementation advice Includes supplementary material: sn.pub/extras

Inhalt

Ames Test (Bacterial Reverse Mutation Test): Why, When, and How to Use.- The Ames II and Ames MPF Penta I Assay: A Liquid Microplate Format Modification of the Classic Ames Test.- Revised Procedure of the Bacterial Reverse Mutation Test for Genotoxic Evaluation of Nanoparticles.- The Control of Hydrophobic Compound Exposure in In Vitro Tests for Genotoxicity.- The In Vitro Micronucleus Assay and FISH Analysis.- The In Vivo Rodent Micronucleus Test.- Chromosomal Aberration Test Utilities In Vitro and In Vivo.- Chromosome Painting of Mouse Peripheral Blood and Spleen Tissues.- T-Cell Receptor Mutation Assay for Monitoring Human Genotoxic Exposure.- The Human RBC PIG-A Gene Mutation Assay.- The Applicable Use of the HPRT Gene Mutation Assay as a Practical Tool in Mutagenesis and DNA Repair Studies.- The Comet Assay: High Throughput Use of FPG.- The Comet Assay In Vivo in Humans.- Analysis of Nanoparticle-Induced DNA Damage by the Comet Assay.- The Comet Assay in Drosophila: Neuroblast and Haemocyte Cells.- The SMART Assays of Drosophila: Wings and Eyes as Target Tissues.- Testing the Genotoxic Potential of Nanomaterials Using Drosophila.- Transgenic Rodent Gene Mutation Assay in Somatic Tissues.- The Mouse Lymphoma Assay.- Bhas 42 Cell Transformation Assay for Genotoxic and Non-Genotoxic Carcinogens.- Methods for Measuring DNA Repair: Introduction and Cellular Repair.- A Standardized Protocol for the In Vitro Comet-Based DNA Repair Assay.- Use of the Comet Assay to Study DNA Repair in Drosophila melanogaster.- Use of RNA Interference to Study DNA Repair.- The LacZ Plasmid-Based Transgenic Mouse Model: An Integrative Approach to Study the Genotoxicity of Nanomaterials.