Focused on the discovery of precise molecular targets for the development of the cancer preventive agents, Cancer Prevention: Dietary Factors and Pharmacology provides researchers and non-researchers with practical methodologies for developing and validating small molecule and phytochemical-derived drug discovery and mechanisms by which these compounds can modulate distinct target proteins involved in oncogenic signaling. While this volume is primarily focused toward cancer prevention research, the range of techniques demonstrated in the book also provides an introduction of cancer prevention research methods to researchers outside the field. Chapters deal with a critical discussion of both laboratory and clinical topics, with each chapter containing both a discursive section along with a detailed methods section. As part of the Methods in Pharmacology and Toxicology series, this meticulous volume includes the kind of key implementation advice that seeks to ensure successful results in the lab.

Practical and authoritative, Cancer Prevention: Dietary Factors and Pharmacology aims to guide research toward identifying molecular targets and conducting human studies with phytochemicals which would, ideally, provide an enhanced approach to the goal of personalized cancer prevention.

Provides practical methodologies for exploring cancer preventive agents Accessible for experienced researchers as well as non-researchers Features detailed methods as well as critical discussions of both laboratory and clinical topics

Inhalt

Combining Computational and Experimental Methods for Identifying Molecular Targets of Phytochemicals.- Common Methods Used for the Discovery of Natural Anticancer Compounds.- Nrf2-Target Approaches in Cancer Chemoprevention Mediated by Dietary Phytochemicals.- Methods to Analyze Chemopreventive Effect of Silibinin on Prostate Cancer Biomarkers Protein Expression.- An Approach to the Evaluation of Berries for Cancer Prevention with Emphasis on Esophageal Cancer.- The Use of Mouse Models for Lung Cancer Chemoprevention Studies.- The Azoxymethane Plus Dextran Sulfate Sodium-Induced Mouse Colon Cancer Model for the Study of Dietary Chemoprevention of Inflammation-Associated Carcinogenesis.- The Use of Seahorse Extracellular Flux Analyzer in Mechanistic Studies of Naturally-Occurring Cancer Chemopreventive Agents.- Utilizing RNA-Seq to Define Phytochemical-Induced Alterations in Insulin and IGF Regulated Transcriptomes.- The Ex Vivo Use of Keratinocytes from Adult Mice to Define Stem Cell Activities in Cancer Research.- Quantitation of Acetaldehyde-DNA Adducts: Biomarkers of Alcohol Consumption.- Imaging Tools in Discovery and Development of Phytochemical Chemopreventive Agents.- Designing the Chemoprevention Trials of Tomorrow: Applying Lessons Learned from Past Definitive Trials.