This volume explores the latest experimental and clinical protocols used to study hepatic encephalopathy (HE). The chapters in this book are organized into three parts, each focusing on different aspects of HE research. Part One covers protocols for diagnosing and monitoring HE in clinical practice. Part Two talks about various animal models and experimental techniques designed to investigate the pathogenesis of HE and evaluate potential treatments. Part Three looks at the underlying mechanisms and pathophysiological processes involved in HE, and presents advanced techniques and strategies for investigating the complex interactions that contribute to HE. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Experimental and Clinical Methods in Hepatic Encephalopathy Research provides researchers and clinicians with a comprehensive guide of both experimental and clinical methods, and the mechanisms underlying the complex condition of HE.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Psychometric Methods for Diagnosing and Monitoring Minimal Hepatic Encephalopathy.- Auditor P300 Event-Related Potentials as a Neurophysiological Marker for Detecting Minimal Hepatic Encephalopathy.- Magnetic Resonance Imaging Quantification of Steatosis, Iron, and Fibrosis in Chronic Liver Disease.- Neuroimaging Applications in Hepatic Encephalopathy: Acquisition Protocols and Basic Preprocessing Techniques.- Immunoenzymatic Assays to Target Central and Peripheral Inflammatory Markers in Preclinical and Clinical Studies of Hepatic Encephalopathy.- Animal Models of Hepatic Encephalopathy.- Neurobehavioral Tests in Preclinical Studies of Hepatic Encephalopathy.- Human Liver Spheroids from Peripheral Blood: A New Insight Approach for Hepatic Encephalopathy Research.- Stereological Estimation of Purkinje Cell Number in Nissl-Stained Sections of Mouse Cerebellum.- Gut Microbiota-Based Strategies for Targeting Hepatic Encephalopathy.- Specialized Established Techniques foe Assessing Multiorgan Damages at Different Levels of the Gut-Live-Brain Axis in Experimental Animal Models of Hepatic Encephalopathy.- Methods for Measuring Brain Mitochondrial Impairment and Oxidative Stress in Hepatic Encephalopathy.- Nessler Histological Staining for Ammonia Detection in Formalin-Fixed Paraffin-Embedded (FFPE) Mammalian Tissue.