This volume details a wide range of basic and advanced techniques associated with research on isolation of important soil protozoa, and identification. Chapters guide readers through calculations of soil protists, basic techniques for isolation, enumeration, enrichment, molecular techniques, and staining techniques.

Authoritative and cutting-edge, Practical Handbook on Soil Protists aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Soil sampling, processing and storage.- Initial observation of protist from soil.- Abundance calculation of naked and testate amoebae from soil.- Abundance calculation of mycophagous amoebae.- Abundance calculation of ciliates from soilAbundance calculation of flagellates from soil.- Methodology to study species diversity of naked amoeba and testate amoeba.- Non-flooded Petri plate method to study the species diversity of flagellates.- Enumeration of testate amoeba through direct count from soil.- Most Probable Number (MPN) method for enumeration of soil protists.- Standard solutions and media used for isolation of soil protists.- Isolation techniques for amoeba.- Isolation of ciliates and flagellates from soil.- Isolation and Enumeration of Mycophagous Protist.- Media used for enrichment of soil protists.- Enrichment of naked amoebae species.- Enrichment of ciliates and flagellates.- Enrichment of mycophagous protists.- Mono-axenic cultivation of protists.- Axenic cultivation of soil protists.- Classical wet mount method for observing live protists.- SEM: Sample Preparation, Fixation and Staining of Protists.- TEM: Sample Preparation, Fixation and Staining of Protists.- Protargol staining technique for identification of soil protists.-FLUTAX Staining of cilliates.- DNA extraction from trophozoites and cysts using manual protocol.- DNA isolation from single ciliate isolate.- Species identification through sequencing.- DNA Barcoding techniques for Protist.- Unequivocal identification through fluorescence in situ hybridization (FISH) technique.- Isolation, DNA extraction, amplification, and gel electrophoresis of single-celled non-marine foraminifera (Rhizaria).- Isolation and cultivation and staining of Paramecium.- Isolation and cultivation of Euglena.