This volume details experimental research with applicable models to study physiology, biochemistry, and molecular biology of the blood-brain barrier (BBB). Chapters guide readers through the physiology of BBB, in vitro cell models to study the BBB, in vivo and ex vivo models to evaluate BBB, permeability, influx, efflux transportation, drug delivery through the BBB, invasive and non-invasive imaging techniques to study BBB; and molecular biomarkers. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Authoritative and cutting-edge *, Blood-Brain Barrier ***** aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

An Overview of the Blood-Brain Barrier.- Differentiation of brain mural cells from human pluripotent stem cell-derived neural crest by activation of Notch3 signaling.- In vitro 2D and 3D cell models of the human blood-brain barrier: demonstrating the beneficial influence of shear stress on brain microvascular endothelial cell phenotype.- Emerging human choroid plexus organoid models for bloodCSF barrier research.- Drosophila as a model to study the blood-brain barrier. -Exploring the use of zebrafish in the blood-brain barrier research.- Evaluation and quantification of blood-brain barrier permeability in a photothrombotic juvenile rodent stroke model using in-vivo MRI and histology.- Transepithelial/transendothelial electrical resistance (TEER) to measure the integrity of blood-brain barrier.- Cell penetrating peptide-decorated nanovectors for nose-to-brain delivery - a new treatment option for CNS diseases.- Microbial Translocation of the Blood-Brain Barrier.- Microdialysis sampling in a free-moving mouse for blood-brain barrier studies.- Methods of Delivering Molecules Through the Blood-Brain Barrier for Brain Diagnostics and Therapeutics.- Transport across the choroid plexus: How to culture choroid plexus epithelial cells and establish a functional assay system.- Increasing BBB Permeability via Focused Ultrasound and Microbubbles: Current Methods in Pre-Clinical Research.- Recording leukocyte rolling and adhesion on meningeal vessels by intravital microscopy.- Molecular Imaging of Blood-Brain Barrier Permeability in Preclinical Models Using PET and SPECT.- Biomarkers for Microvascular Proteins Detection: Blood-Brain Barrier Injury and Damage Measurement (IH, IF, EM, and Quantification of Tight Junction Integrity).- Deep learning and machine learning algorithms: A Blood Brain Barrier Permeability Prediction Model with Accuracy.