This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.

Provides new insights into activity-based protein profiling (ABPP), which uses chemical probes to profile the functional state of enzymes in native biological systems Focuses on the application of ABPP methodologies to processes relevant to microbiology and immunology Helps readers identify the posttranslational regulatory mechanisms that modulate protein function

Autorentext
Benjamin F. Cravatt, Ph.D.

Department of Chemistry

The Scripps Research Institute

La Jolla, CA, USA

cravatt@scripps.edu

Ku-Lung Hsu Ph.D.

University of Virginia School of Medicine

Department of Chemistry

Charlottesville, VA, USA

kh4ch@virginia.edu

Eranthie Weerapana Ph.D.

Boston College

Merkert Chemistry

Chestnut Hill, MA, USA

eranthie@bc.edu

Inhalt

Activity-based protein proling enabling multimodal functional studies of microbial communities.- Activity-Based Protein Proling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles.- Opportunities and Challenges in Activity-Based Protein Proling of Mycobacteria.- Activity-Based Protein Proling at the HostPathogen Interface.- Chemical Proteomic Proling of Protein Fatty-Acylation in Microbial Pathogens.- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling.- ABPP and HostVirus Interactions.- Activity-Based Protein Proling for the Study of Parasite Biology.- Deciphering T Cell Immunometabolism with Activity-Based Protein Proling.- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics.- Development of Activity-Based Proteomic Probes for Protein Citrullination.- Recent Advances in Activity-Based Protein Proling of Proteases.- Opportunities for Lipid-Based Probes in the Field of Immunology.- Activity-Based Protein Proling of Non-ribosomal Peptide Synthetases.- Target Identication of Bioactive Covalently Acting Natural Products.- Applications of Reactive Cysteine Proling.