This volume covers a range of fundamental and advanced techniques and methods used by researchers to study the control of yellow fever. The chapters in this book cover a broad range of topics including epidemiological studies that track the spread of disease and sophisticated laboratory techniques that investigate the disease's pathogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Yellow Fever Virus: Methods and Protocols is a valuable guide for students, researchers, and health professionals who are looking to understand and combat yellow fever. This book will inspire new discoveries, collaborations, and initiatives that will bring humanity closer to eradicating this disease.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Flow Cytometry as Immunoassay Tool for Research on Yellow Fever Virus.- Clinical Features of Human Infection by Yellow Fever.- ELISA for Detection of Total IgG against YF 17DD.- A Fast RT-qPCR One-Step to Quantify Yellow Fever Virus.- Immunoenzyme Test for Detection of IgG Anti-YFV Class Antibodies IgG-ELISA.- Immunofluorescence Assay.- Type I Interferon-Induced Modification of Yellow Fever Virus NS5 Enhances Binding to Human STAT2.- Neutralization Tests for Quantification of Specific Antibodies Against Yellow Fever Virus.- Purification of Yellow Fever Virus by Liquid Chromatography.- Conventional RT-PCR for Yellow Fever Virus.- Sequencing of Yellow Fever Virus.- Detection of Viral Particles in Paraffinized Tissued and Cell Culture by Indirect Immunofluorescence.- Yellow Fever Virus Genotyping Tool Using RT-qPCR Followed by Nucleotide Sequencing and Phylogenetic Analysis.- Detection of Viral RNA in Paraffinized Tissues by Nested RT-PCR.- Automation on the Plate Analysis from Yellow Fever Serum Neutralization Assays.- NS5 Region Cloning Protocol, For the Construction of an Accurate Standard Curve in the Detection and Diagnosis of the Yellow Fever Virus.- Micro-Focus Reduction Neutralization Tests (mFRNT) for Neutralizing Antibody (Nab) Quantification against Yellow Fever Virus.- Quantification of Yellow Fever Virus by Plaque Assay and Focus Formation Assay.- Thawing, Growth, and Maintenance of Vero Cell Lines.- Yellow Fever Virus Bank Production in Stationary Cell Culture.- Yellow Fever Virus Production in Bioreactor.- Immunoenzyme Test for Capture of IgM Anti-YFV Class Antibodies MAC-ELISA.- Enhancing Biosafety Protocols in Laboratories Conducting Research on the Yellow Fever Virus.- Epidemic Outbreaks Related to Yellow Fever Viruses.