Welcome to your study of enzyme kinetics, the subject that underlies all enzymology, which in turn underlies all aspects of biochemistry. This text will give you an introduction to a wide range of topics that constitute the modern enzyme kinetics. This textbook is directed at graduate students in biochemistry, chemistry, and life sciences, for advanced courses in enzyme kinetics, enzymology, and enzyme chemistry. For this reason, the whole book is organized in a systematic and scholarly fashion. It is unlikely that the student will be expected to cover everything in the text, but in a later career she or he may find it an invaluable reference for topics that are needed in practice. The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory. The preparation of the manuscript for this book was under the constant surveillance of W. Wallace Cleland, Professor of Chemical Science at the University of Wisconsin in Madison, and one of the founders of modern enzyme kinetics. Without his help and advice, this bookwould not be possible. Several versions of the manuscript were constantly corrected and improved by Svetlana Professor of Biochemistry at the University of Novi Sad.

Provides a systematic introduction to the field of enzyme-catalyzed reactions Outlines the subject in such a way that it builds from less complicated to more demanding kinetic models

Klappentext

This book provides a systematic introduction to the field of enzyme-catalyzed reactions. The content develops from monosubstrate to bisubstrate to trisubstrate reactions, concluding with nonhyperbolic rate equations and allosteric and cooperative effects. Because it outlines the subject in such a way that it builds from less complicated to more demanding kinetic models, it can be used as a textbook for students of biochemistry and molecular biology. The author stresses the importance of graphical representation of kinetic models by frequent use of such mathematical models in the form of double-reciprocal plots. In addition, special attention is paid to isotope exchange studies, kinetic isotope effects, and the statistical evaluation of initial rate and ligand binding data. TOC:STRONG1./STRONG Introduction.- STRONG2./STRONG Chemical kinetics.- STRONG3./STRONG Kinetics of monosubstrate reactions.- STRONG4./STRONG Derivations of rate equations.- STRONG5./STRONG Linear inhibition.- STRONG6./STRONG Hyperbolic and parabolic inhibition.- STRONG7./STRONG Enzyme activation.- STRONG8./STRONG Kinetics of rapid equilibrium bisubstrate reactions.- STRONG9./STRONG Steady state kinetics of bisubstrate reactions.- STRONG10./STRONG Kinetic analysis of bisubstrate mechanisms.- STRONG11./STRONG Substrate inhibition and mixed dead-end and product inhibition.- STRONG12./STRONG Kinetics of trisubstrate reactions.- STRONG13./STRONG Cooperative and allosteric effects.- STRONG14./STRONG The pH dependence of enzyme catalysis.- STRONG15./STRONG Effects of temperature on enzyme reactions.- STRONG16./STRONG Isotope exchange.- STRONG17./STRONG Solvent and kinetic isotope effects.- STRONG18./STRONG Statistical analysis of initial rate and binding data.- Subject index.

Inhalt
Chemical Kinetics.- Kinetics of Monosubstrate Reactions.- Derivation of Rate Equations.- Linear Inhibition.- Hyperbolic and Parabolic Inhibition.- Enzyme Activation.- Kinetics of Rapid Equilibrium Bisubstrate Reactions.- Steady-State Kinetics of Bisubstrate Reactions.- Kinetic Analysis of Bisubstrate Mechanisms.- Substrate Inhibition and Mixed Dead-End and Product Inhibition.- Trisubstrate Mechanisms.- Cooperative and Allosteric Effects.- The pH Dependence of Enzyme Catalysis.- Effects of Temperature on Enzyme Reactions.- Isotope Exchange.- Kinetic Isotope Effects.- Statistical Analysis of Initial Rate and Binding Data.