Viral Applications of Green Fluorescent Protein

This book detailing various applications of fluorescent proteins delivered by viruses contains lab protocols that enable researchers to create rapid, readily reproducible results. It also highlights tips on troubleshooting and how to avoid pitfalls.

Over the last ten years, Green Fluorescent Proteins, along with the other spectral variants, have emerged from near obscurity to become a powerful and versatile tool in scientific research. In Viral Applications of Green Fluorescent Protein: Methods and Protocols , leading investigators from around the world contribute detailed examples of both the construction and application of fluorescent proteins delivered by viruses in a format crafted to produce rapid, readily reproducible results. Written in the style of the popular and successful Methods in Molecular Biology™ series, the chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Viral Applications of Green Fluorescent Protein: Methods and Protocols supplies researchers with an ideal guide to the many uses of GFP and a vital starting point for future studies utilizing this highly adaptable protein.

Contains protocols useful for both basic researchers and for those interested in applied viral gene therapy Includes viral methods for terminally differentiated cells and for undifferentiated cells, including human stem cells Provides example applications for many different viral vectors including HIV, herpes virus, pox virus, cytomegalovirus, adenovirus and adeno-associated viruses, baculovirus, SARS, and many others Examines state-of-the-art imaging techniques including in vivo two-color fluorescence video micrsocopy and the combination of fluorescence imaging with PET, SPECT or MRI Describes viral applications applicable for Medicine, Neuroscience, Immunology, Agriculture and Laboratory Safety

Inhalt
Using Fluorescent Proteins to Study Poxvirus Morphogenesis.- Molecular Imaging of Human Embryonic Stem Cells.- Use of a GFP-PML-Expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection.- Dual-Color Imaging of Tumor Angiogenesis.- Use of GFP to Analyze Morphology, Connectivity, and Function of Cells in the Central Nervous System.- Expression of EGFP by Adenovirus-Mediated Gene Transfer in the Central Nervous System.- Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes.- Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry.- Production of Multicistronic HIV-1 Based Lentiviral Vectors.- Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps.- Use of a Macrophage-Tropic GFP-Tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs.- GFP-Lentiviral Vectors Targeting for NeuroAIDS.- Construction of Cell-Type Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery.- Selective Protein Expression Within the CNS Using Hybrid Lentivirus.- Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein.- The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication.- Modified Bovine Herpesvirus 1 for Protein Secretion.- Identification of Viral Peptide Fragments for Vaccine Development.- Use of GFP to Investigate Expression of Plant-Derived Vaccines.- Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions.- Sendai Virus for Cancer Immunotherapy.- Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells.- Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity.- Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells.