Summarizes transgenesis techniques in ascidians and up-to-date concrete research using those techniques

Enables readers to quickly access and compare techniques at a glance

Helps readers to apply the strategies contained here to their own studies

Autorentext
Yasunori Sasakura started his research career in Dr. Noriyuki Satoh's laboratory in 1996. His Ph.D. thesis was about the localization of mRNAs in eggs of the ascidian Halocynthia roretzi. He then focused on developing the genetic approaches in the ascidian Ciona intestinalis. The reason is that he thought that genetics would be necessary to elucidate molecular mechanisms of maternally stored substances. He introduced transposon-mediated germ cell transgenesis and mutagenesis in this ascidian. From then on he brought many technical innovations in the study of Ciona with transgenic techniques. For example, his group has introduced transgenesis-mediated tissue/cell tracing with the photoconvertible protein Kaede, transposon-based specific knockdown of maternally transcribed RNAs, the optogenetic approach to understand neuronal functions, and genome editing techniques in Ciona with TALENs and CRISPR/Cas9.

Inhalt

Foreword.-Preface.-Chapter 1. Introduction chapter.-Chapter 2. Microinjection of exogenous nucleic acids into eggs: Ciona species.-Chapter 3. Practical guide for the ascidian microinjection: Phallusia mammillata .-Chapter 4. Microinjection of exogenous DNA into eggs of Halocynthia roretzi .-Chapter 5. Electroporation in ascidians: history, theory and protocols.-Chapter 6. The use of cis -regulatory DNAs as molecular tools.-Chapter 7. Reporter Analyses Reveal Redundant Enhancers that Confer Robustness on Cis -Regulatory Mechanisms.-Chapter 8. Investigating evolutionarily conserved molecular mechanisms controlling gene expression in the notochord.-Chapter 9. Purification of Fluorescent Labeled Cells from Dissociated Ciona Embryos.- Chapter 10. Germline transgenesis in Ciona Germline transgenesis in Ciona .-Chapter 11. The enhancer trap in Ciona .-Chapter 12. TALEN-based knockout system.-Chapter 13. CRISPR knockouts in Ciona embryos.-Chapter 14. Transgenic techniques for investigating cell biology during//