The Organization of Cell Metabolism

The NATO Advanced Research Workshop on nThe Organization of Cell n Metabolism was held 31 August - 4 September, 1985, at the Hotel Hansthol m in Hansthollllt Denmark. From areas of cell biology, bio chemistry, enzymology, and biophysical chemistry, the Workshop brought together workers whose research focuses on the character of the metabolic infrastructure of the living cell. The organizing committee was composed of the following members: James S. Clegg Douglas B. Kell Paul A. Srere G. Rickey Welch (Chairman) There has now arisen an edifice of proof that cell metabolism is highly ordered in space and time. The refinement of cytological extraction methodology and the development of more gentle isolation techniques have led to the demonstration of structural organization for most of the primary metabolic pathways. Moreover, the study of isolated enzyme complexes and membranous arrays has revealed unique functional properties of the organized state. Interest in metabolic organization has heightened very recently, due to the emergence of exciting new cytochemical and electron-microscopic advances in the elucidation of the cytOOIatrix and associated cell water. The hyaloplasmic space of eukaryotic cells has now been shown to be laced with a dense network of various filamentous structures - one role of which appears to be that of a structural support for the microcompartmentation of metabolic processe~ The supramolecular organization of certain processes may exceed the physical confines of intracellular particulates, involving large-scale entrainment of the cytoplasm in space and time.

Inhalt
The Organization of Cell Metabolism: A Historical Vignette.- Organization of The Cytomatrix and Aqueous Compartments.- Structural Organization of the Cytomatrix.- The Cytoskeleton.- Interactions within the Cytoskeleton.- On the Physical Properties and Potential Roles of Intracellular Water.- Diffusion of a Small Molecule in the Aqueous, Compartment of Mammalian Cells.- Restricted Motion of Cellular K+ in Permeabilized Cells.- The Intracellular Distribution of Adenosine Triphosphate.- Organization of Macromolecular Synthesis.- New Views of Cell and Tissue Cytoarchitecture: Embedment-Free Electron Microscopy and Biochemical Analysis.- Understanding the Organization of Cell Metabolism in Early Embryonic Systems: Developmental Implications.- Organization of Biosynthetic And Biodegradative Processes.- Organization of Enzymes in the Tryptophan Pathway.- Implications of Metabolic Compartmentation in Prokaryotic Cells.- Organization of Glucose Metabolism: A Model of Compartments by Poly-Isozymic Complexes.- Direct Transfer of Metabolites via Enzyme-Enzyme Complexes: Evidence and Physiological Significance.- Energetic Consequence of Dynamic Enzyme-Enzyme Interaction.- Mitochondrial Hexokinase: Interactions between Glycolysis and Oxidative Phosphorylation.- Diffusion and Perfusion in the Living Cell: Implications for Metabolic Regulation and Organization.- Organization of Energy Metabolism: Enzymological Approaches.- Interactions of Mitochondrial Matrix Enzymes with Mitochondrial Inner Membranes.- Some Aspects of Enzyme Organization in the Citric Acid Cycle.- Enzyme Interactions in the Citric Acid Cycle and the Aspartate-Malate Shuttle.- EPR Measurements of Conformational Changes on - the E1 Component of the Pyruvate Dehydrogenase Complex from Esoheriohiacoli.- Organization of Energy Metabolism: Im Situ Approaches.- Organization and Control of Energy Metabolism in Anaerobic Microorganisms.- Electron and Proton Flow in Hepatocytes during Fatty Acid and Pyruvate Oxidation: Implications for Energy Transduction and Heat Production.- Experimental Analysis of Spatiotemporal Organization of Metabolism in Intact Cells: The Enigma of Metabolic Channeling and Metabolic Compartmentation.- Functional and Structural Heterogeneity of the Inner Mitochondrial Membrane.- Experimental and Theoretical Modeling of Metabolic Organization.- Complex Patterns of Excitability and Oscillations in a Biochemical System.- Reaction-Diffusion Coupling in Immobilized Enzyme Systems: Ionic Interactions.- On Parts and Wholes in Metabolism.- Maxwell's Demons in Channelled Metabolism: Paradoxes and Their Resolution.- Molecular Machines and Energy Channelling.- Cytosociological Aspects of Enzyme Action.- Participants.