Somatic Cell Genetics

This book represents a selected group of manuscripts from lecturers participating in the NATO/Gulbenkian Foundation sponsored course on Somatic Cell Genetics held May 31 to June 12, 1981 in the Hotel Montechoro in the Algarve of Portugal. The text will provide those students who could not attend the meeting with a current survey of important advances in the field of Somatic Cell Genet ics. It is not possible to recapture here all the lectures, semi nar discussions, student and faculty interactions, the ambience of the Algarve and the time devoted exclusively to scientific discus sion. In summary, I feel that this book is good, but the scien tists, the students, and the entire course were better. Somatic Cell Genetics is a broad subject area and one which has contributed significantly to Our understanding of the mammalian cell. Drs. Caskey, Buttin, Siminovitch, and Lechner elected in designing the course to focus on the results obtained with cultured animal cells.

Inhalt
Pathway Mutants in Mammalian Cells and their Contribution to the Analysis of Drug Resistance.- Isolation and Analysis of Chinese Hamster Cells Carrying Forward and Reverse Mutations in the Hypoxanthine-Guanine Phosphoribosyltransferase Locus.- Towards Mapping Genes to Chromosomes Using Two Dimensional Gel Electrophoresis of Proteins and Somatic Cell Hybridization. Preliminary Studies.- The Use of Mouse-Human and Human-Human Hybridomas in Human Genetics and Immunology.- Expression of Liver Mono-Oxygenase Functions Induced by Xenobiotics.- Gene Mapping and Gene Transfer in Mammalian Cells.- Dihydrofolate Reductase Gene Amplification, Altered Dihydrofolate Reductase, and Methotrexate Resistance in Cultured 3T6 Cells Associated with Unstable Amplification of an Altered Dihydrofolate Reductase Gene.- Transformation and Expression of TK Sequences.- Genetic Variants of Cultured Animal Cells.- Cell Hybridization: A Tool for the Study of Cell Differentiation.- Use of Somatic Cell Hybridization and DNA-Mediated Gene Transfer for Characterization of Neoplastic Transformation.- Analysis of Cell Surface Antigens with Monoclonal Antibodies.- List of Contributors.