RNA Scaffolds

This volume provides techniques on recent developments that use RNA scaffolds as molecular tools. RNA Scaffolds: Methods and Protocols guides readers through methods on various domains as molecular biology, nanotechnologies, and structural biology. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, RNA Scaffolds: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
A Method to Predict the 3D Structure of an RNA Scaffold.- Post-crystallization Improvement of RNA Crystal Diffraction Quality.- Expression and Purification of RNA-protein Complexes in Escherichia coli.- Production of Homogeneous Recombinant RNA using a tRNA Scaffold and Hammerhead Ribozymes.- In vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold.- Detection of RNA-Protein Interactions using Tethered RNA Affinity Capture.- A Universal Method for Labeling Native RNA in Live Bacterial Cells.- Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors .- RNA Scaffold: Designed To Co-Localize Enzymes.- Artificial Ligase Ribozymes Isolated by a Design And Selection Strategy.- Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach.- Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells.- Design and Characterisation of Topological Small RNAs.- Folding RNA-Protein Complex into Designed Nanostructures.- Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60 to 90 or 108.- RNA-Mediated CdS Based Nanostructures.- An Effective Method for Specific Gene Silencing in Escherichia coli using Artificial Small RNA.