RNA and DNA Editing

Covering many state-of-the-art techniques, this book features leading experts in the field who introduce methods developed in recent years to study editing substrates, mechanisms of specificity, and the functions of RNA- and DNA-editing enzymes and complexes.

The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols , leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei , adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.

Features cutting-edge methodologies focused on the study of this diverse field of study Includes unique genetic, molecular, biochemical, and computational approaches Contains expert tips and implementation advice Includes supplementary material: sn.pub/extras

Inhalt

Three-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy.- iCODA: RNAi-Based Inducible Knock-In System in Trypanosoma brucei .- Perturbing A-to-I RNA Editing Using Genetics and Homologous Recombination.- Laser Microdissection and Pressure Catapulting of Single Human Motor Neurons for RNA Editing Analysis.- Biochemical Identification of A-to-I RNA Editing sites by the Inosine Chemical Erasing (ICE) Method.- Identifying mRNA Editing Deaminase Targets by RNA-Seq.- Mouse and Other Rodent Models of C to U RNA Editing.- In vivo Analysis of RNA Editing in Plastids.- Identifying Specific Trans-Factors of RNA Editing in Plant Mitochondria by Multiplex Single Base Extension Typing.- Complementation of Mutants in Plant Mitochondrial RNA Editing by Protoplast Transfection.- A High Throughput Assay for DNA Deaminases.- Biochemical Fractionation and Purification of High-Molecular-Mass APOBEC3G Complexes.- Analysis of tRNA Editing in Native and Synthetic Substrates.- Post-Transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs.- Functional Analysis of Non-Coding RNAs in Trypanosomes: RNA Walk, A Novel Approach to Study RNA-RNA Interactions Between Small RNA and Its Target.- A Post-Labeling Approach for the Characterization and Quantification of RNA Modifications Based on Site-Directed Cleavage by DNAzymes.