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RNA and DNA Editing

  • Kartonierter Einband
  • 292 Seiten
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Covering many state-of-the-art techniques, this book features leading experts in the field who introduce methods developed in rece... Weiterlesen
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Beschreibung

Covering many state-of-the-art techniques, this book features leading experts in the field who introduce methods developed in recent years to study editing substrates, mechanisms of specificity, and the functions of RNA- and DNA-editing enzymes and complexes.


The recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues. In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.

Features cutting-edge methodologies focused on the study of this diverse field of study

Includes unique genetic, molecular, biochemical, and computational approaches

Contains expert tips and implementation advice



Zusammenfassung
Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications.

Inhalt

Part I: Uracil Insertion/Deletion RNA E diting in M itochondrion of Trypanosoma brucei

1. Three-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy

H. Ulrich Göringer, Holger Stark, Cordula Böhm, Bjoern Sander, and Monika M. Golas

2. iCODA: RNAi-Based Inducible Knock-In System in Trypanosoma brucei

Gene-Errol Ringpis, Richard H. Lathrop, and Ruslan Aphasizhev

Part II: A denosine to I nosine RNA E diting

3. Perturbing A-to-I RNA Editing Using Genetics and Homologous Recombination

Cynthia J. Staber, Selena Gell, James E.C. Jepson, and Robert A. Reenan

4. Laser Microdissection and Pressure Catapulting of Single Human Motor Neurons for RNA Editing Analysis

Hui Sun, Mary A. O'Connell, Valerie Mann, Brendon Noble, and Liam P. Keegan

5. Biochemical Identification of A-to-I RNA Editing sites by the Inosine Chemical Erasing (ICE) Method

Masayuki Sakurai and Tsutomu Suzuki

Part III: Cytidine to Uracil RNA and DNA E diting

6. Identifying mRNA Editing Deaminase Targets by RNA-Seq

Brad R. Rosenberg, Scott Dewell, and F. Nina Papavasiliou

7. Mouse and Other Rodent Models of C to U RNA Editing

Valerie Blanc and Nicholas O. Davidson

8. In vivo Analysis of RNA Editing in Plastids

Stephanie Ruf and Ralph Bock

9. Identifying Specific Trans-Factors of RNA Editing in Plant Mitochondria by Multiplex Single Base Extension Typing

Mizuki Takenaka

10. Complementation of Mutants in Plant Mitochondrial RNA Editing by Protoplast Transfection

Mizuki Takenaka and Anja Zehrmann

11. A High Throughput Assay for DNA Deaminases

Meng Wang, Cristina Rada, and Michael S. Neuberger

12. Biochemical Fractionation and Purification of High-Molecular-Mass APOBEC3G Complexes

Ya-Lin Chiu

Part IV: tRNA Editing and RNA M odifications

13. Analysis of tRNA Editing in Native and Synthetic Substrates

Jessica L. Spears, Kirk W. Gaston, and Juan D. Alfonzo

14. Post-Transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs

Chao Huang, John Karijolich, and Yi-Tao Yu

15. Functional Analysis of Non-Coding RNAs in Trypanosomes: RNA Walk, A Novel Approach to Study RNA-RNA Interactions Between Small RNA and Its Target

Chaim Wachtel and Shulamit Michaeli

16. A Post-Labeling Approach for the Characterization and Quantification of RNA Modifications Based on Site-Directed Cleavage by DNAzymes

Madeleine Meusburger, Martin Hengesbach, and Mark Helm

Produktinformationen

Titel: RNA and DNA Editing
Untertitel: Methods and Protocols
Editor:
EAN: 9781493958214
ISBN: 1493958216
Format: Kartonierter Einband
Herausgeber: Humana Press
Genre: Medizin
Anzahl Seiten: 292
Gewicht: 554g
Größe: H254mm x B178mm x T15mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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