This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Reverse Genetics for Mammalian Orthoreovirus.- Development and Characterization of an Infectious cDNA Clone of Equine Arteritis Virus.- Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus.- Reverse Genetics of Zika Virus.- Efficient Reverse Genetic Systems for Rapid Genetic Manipulation of Emergent and Pre-Emergent Infectious Coronaviruses.- Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus.- Rescue of Sendai Virus from Cloned cDNA.- BAC-based Recovery of Recombinant Respiratory Syncytial Virus (RSV).- Recovery of a Paramyxovirus, the Human Metapneumovirus, from Cloned cDNA.- Reverse Genetics of Newcastle Disease Virus.- Reverse Genetics Systems for Filoviruses.- Rapid Reverse Genetics Systems for Rhabdoviruses- From Forward to Reverse and Back Again.- Lassa Virus Reverse Genetics.- Reverse Genetics of Influenza B Viruses.- Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA.- Plasmid-based Reverse Genetics of Influenza a Virus.