This detailed volume explores quantum dot (QD) techniques, spanning from materials preparation and surface functionalization to bioimaging and sensing. Split into four sections, the book covers the preparation of different types of QDs, including binary and ternary compositions, tutorials on using computational tools to optimize optical properties and enable single-QD tracking, the functionalization of QDs for bioimaging and cellular labeling, as well as the application of QDs for biosensing and biomarker detection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Up-to-date and practical, Quantum Dots: Applications in Biology, Fourth Edition serves as a bridge between fundamental research on QDs and impactful applications in the life sciences.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Synthesis of PbS/CdS/ZnS Core/Shell/Shell Quantum Dots for Multiplexed Shortwave Infrared Imaging.- Preparation of BSA-CuxS Quantum Dots via a One-Pot Synthesis Approach Using the Biuret's Reaction.- Synthesis of Environmentally Friendly Heavy-Metal-Free AgInS2/ZnS Quantum Dots Using Microwave Heating.- Preparation of CuInS2 Quantum Dots with Reduced Toxicity for Biological Applications.- Preparation of Bimodal Nanoprobes for Fluorescent and Magnetic Resonance Imaging by Conjugating Gadolinium Complexes to Quantum Dots.- Deep Learning Tools for Single Quantum Dot Tracking.- Machine Learning for Optimizing the Optical Properties of Quantum Dots.- Imaging and Reconstruction of Three-Dimensional Quantum Dot Trafficking in Live Cells by Spinning Disk Confocal Microscopy.- Supra-Nanoparticle Assemblies of Quantum Dots for Extracellular Immunolabeling.- Precise and Stable Labeling of Quantum Dots with Engineered Antibodies.- Conjugation of Quantum Dots to 3-Mercaptophenylboronic Acid for Analysis of Sialic Acids on Erythrocyte Surfaces.- Preparation of Ag2Te Quantum Dots for Near-Infrared Fluorescence and Computed Tomography Imaging.- Quantum Dot-Based Approaches for Labeling Epithelial Damage in Living and Fixed Zebrafish Embryos.- Quantum Dots for Assessment of ROS Accumulation During Chemotherapy, Radiotherapy, and Chemoradiotherapy.- Quantum Dot-Based Fluorescence-Linked Immunosorbent Assays for the Detection of Biomarkers.- Glutathione-Coated Quantum Dots as Immobilized Substrates for the Evaluation of Glutathione-Dependent Enzymes: Glutathione Reductase and Glutathione S-Transferase.