This detailed book explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields.
Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.

Compiles various biotechnological tools and assays for the detection of both antigens and antibodies Provides technically sound protocols for newer diagnostics tools Contains problems encountered while using diagnostic tools as well as probable solutions with examples

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Dr. Rajib Deb, PhD is currently employed as Scientist at Indian Council of Agricultural Research-National Research Centre on Pig, Rani, Guwahati, Assam, India. He has served more than 9 years at Agricultural Research Service, India. He completed his post-graduation and PhD at Indian Veterinary Research Institute, Uttar Pradesh, India in the subject of Animal Biotechnology. He is currently pursuing the National Post-Doctoral Fellowship program at National Dairy Research Institute, Karnal, Haryana, India. He has published more than 35 research papers in peer reviewed journals. He is the recipient of several National and International Awards including TWAS Fellowship, Italy; Associate of National Academy of Agricultural Sciences, India; Member of National Academy of Sciences, India; Member of Indian National Young Academy of Sciences, India. He has filed 9 Indian Patents and developed several technologies. He is currently working on the development of diagnostics and vaccines against porcine disease. Dr. Ajay Kumar Yadav, PhD is the scientist in veterinary virology at Indian Council of Agricultural Research-National Research Centre on Pig, Rani, Guwahati, Assam, India. He has completed graduation from Govind Ballabh Pant University of Agriculture and Technology, Uttarakhand, India. Later on, he completed his M.V. Sc. and PhD in veterinary virology from the premier veterinary institute named Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, Uttar Pradesh, India. Dr. Yadav joined the Agricultural Research Services (ARS) in the discipline of veterinary microbiology in the year 2018. His research interests include disease diagnosis, cell culture, and virus isolation. He is a member of several societies, and has also published more than 20 research articles in peer-reviewed international and national journals. He has more than 60 GenBank submissions. Dr. Swaraj Rajkhowa, PhD joined Agricultural Research Service (ARS) as Scientist in the year 1999. He completed his Ph.D. from Assam Agricultural University in the year 2005. He did his Post-Doctoral studies at internationally reputed institutes like Cornell University, New York, NY, USA and University of California, Irvine, CA, USA where he learned advanced molecular tools like MLST, SLST, SNP, and DNA Microarray technology. His area of interest is molecular epidemiology and molecular diagnosis of infectious diseases of animal origin, including diseases of public health significance. He has been actively involved in diagnosis and management of livestock diseases over the last 20 years and particularly on porcine diseases for the last 12 years. He has published more than 100 research papers in peer reviewed national and international journals of repute, acted as reviewer of several national and international journals, handled more than 10 externally funded projects as Principal Investigator and several institute-funded projects, guided Masters and doctoral degree students (including post-doctoral scholar), and acted as external examiner of both M.V.Sc. and Ph.D. students of SAUs. Prof. (Dr.) Yashpal Singh Malik, PhD is serving as Dean, College of Animal Biotechnology at Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab, India, and previously served as ICAR-National Fellow at Indian Veterinary Research Institute, Izatnagar, India. He works in viral disease epidemiology, virus-host interactions, microbial biodiversity, characterization, and diagnosis of pathogens. Prof. Malik has acquired advanced training in Molecular Virology from the University of Minnesota, Minneapolis, MN, USA; Division of Virology, University of Ottawa, Ottawa, ON, Canada, and Wuhan Institute of Virology, Wuhan, China. He is a recipient of several prestigious National, State, and Academy awards and honors, including the ICAR-Jawaharlal Nehru Award. He has supervised 5 Ph.D. and 17 M.V.Sc. students. Has authored 5 books, 25 book chapters, and published 217 scientific research and review articles. Dr. Malik has been the Editor-in-Chief of Journal of the Immunology Immunopathology. He has also edited special issues of the SpringerNature journal, VirusDisease, Bentham's journal, Journal of Current Drug Metabolism, on emerging thematic areas.

Inhalt
Requirements and Preparedness for Attending a Viral Disease Outbreak in Pig Farms.- Collection of Samples, Their Preservation, and Transportation.- Methods for Quantification of Viruses.- Protocols for Isolation of Genetic Materials from RNA Viruses.- Multiplex PCR for Diagnosis of Porcine Diseases.- Protocols for Isolation of Plasmid DNA.- Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases.- RNA-PAGE-Based Diagnosis of Viral Diseases.- Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases.- Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases.- Aptamers as Diagnostic Markers for Viral Infections of Veterinary Importance.- Antibody-Based Sensors for Pathogen Detection.- Lateral Flow Assay for Diagnosis of Pig Viral Diseases.- Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases.- Protocols for Immunofluorescence Techniques.- Polymerase Spiral Reaction (PSR) for the Diagnosis of Porcine Viral Diseases.- Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases.- Cell Culture System for Porcine Virus Isolation and Propagation.- An Overview of Mouse Monoclonal Antibody Production.- Nucleic Acid Hybridization Techniques for Viral Disease Diagnosis: A Detailed Perspective.- Ligase Detection ReactionFluorescent Microsphere Assay.- ELISA as a Diagnostic Weapon.- SDS-PAGE and Western Blotting: Basic Principles and Protocol.- Immune Assays as Diagnostic for Pig Viral Diseases.- Production of Virus-Like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis.- Good Laboratory Practices and Biosafety Containments in a Virology Laboratory.