Post-Transcriptional Gene Regulation

This volume aims to provide the most recent advances in techniques for studying gene expression regulation at the post-transcriptional level. Post-Transcriptional Gene Regulation, Second Edition is organized in six sections describing bioinformatics approaches for studying post-transcriptional regulation, various expression profiling approaches, protein-RNA interaction and non-coding RNA investigation techniques, methods for profiling RNA modifications, and other techniques such as alternative translation initiation or polyadenylation sites determination. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Post-Transcriptional Gene Regulation, Second Edition is a versatile resource to researchers studying post-transcriptional regulation, introducing the most recent techniques and providing a comprehensive guide to their implementation.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression.- A Computational Approach for the Discovery of Protein-RNA Networks.- Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells.- Studying the Translatome with Polysome Profiling.- Exploring Ribosome Positioning on Translating Transcripts with Ribosome Profiling.- Studying Isoform-Specific Recruitment to Polyribosomes with Frac-Seq.- Use of the Pbutr Reporter System for Scalable Analysis of 3' UTR-Mediated Gene Regulation.- Comprehensive Identification of RNA-Binding Proteins by RNA Interactome Capture.- Identifying RBP Targets with RIP-Seq.- PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites.- Profiling the Binding Sites of RNA-Binding Proteins with Nucleotide Resolution Using Iclip.- A Pipeline for PAR-CLIP Data Analysis.- Capture and Identification Of Mirna Targets By Biotin Pulldown And RNA-Seq.- Identification of Mirna-Target RNA Interactions Using CLASH.- Genome-Wide Analysis of A-To-I RNA Editing.- Nucleotide-Level Profiling Of M 5 c RNA Methylation.- Probing N 6 -Methyladenosine (M 6 a) RNA Modification in Total RNA with SCARLET.- Genome-Wide Identification of Alternative Polyadenylation Events Using 3'T-Fill.- Genome-Wide Profiling Of Alternative Translation Initiation Sites.- Genome-Wide Study Of Mrna Isoform Half-Lives.- Visualizing Mrna Dynamics in Live Neurons and Brain Tissues.- Single Molecule Live-Cell Visualization of Pre-Mrna Splicing.