Plant Pathology

This volume covers the latest developments in different areas of plant pathology. The chapters in this volume are organized into seven parts. Part One provides traditional methods for isolation and identification of invasive pathogens and root disease. Part Two looks at new and rapid DNA extraction protocols from different samples, and Part Three focuses on molecular detection protocols for identifying and quantifying plant pathogens, including fungal and bacterial invasive species. Part Four describes the application of metabarcoding in plant pathology, and Part Five talks about plant pathogen interactions. Part Six concentrates on population genomics of plant pathogens, and Part Seven covers biocontrol on plant pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Plant Pathology: Methods and Protocols is a valuable resource for researchers in the plant pathology community, and discusses various approaches for the detection, identification, and control of plant diseases.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Obtaining and Maintaining Cultures of Pinewood Nematodes Bursaphelenchus xylophilus from Wild Dauers.- Survey and Monitoring of Phytophthora Species in Natural Ecosystems: Methods for Sampling, Isolation, Purification, Storage, and Pathogenicity Tests.- Field and Laboratory Procedures for Fusarium circinatum Identification and Diagnosis.- Rapid Extraction of Plant Nucleic Acids by Microneedle Patch for In-Field Detection of Plant Pathogens.- DNA Extraction Methods to Obtain High DNA Quality from Different Plant Tissues.- A Rapid Method for Extracting High Quality DNA from Soils.- Invasive Alien Plant Pathogens: The Need of New Detection Methods.- Detection of Airborne Inoculum of Hymenoscyphus fraxineus: The Causal Agent of Ash Dieback.- Molecular Detection of Wheat Blast Pathogen in Seeds.- Detection and Identification of the Causal Agents of Dothistroma Needle Blight.- The Use of qPCR to Detect Cryphonectria parasitica in Plants.- Rapid Molecular Diagnostics in the Field and Laboratory to Detect Plant Pathogen DNA in Potential Insect Vectors.- A Panel of Real-Time PCR Assays for the Direct Detection of All the Xylella fastidiosa Subspecies.- Selective Quantification of Erwinia amylovora Live Cells in Pome Fruit Tree Cankers by Viability Digital PCR.- Innovative Detection of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens, Causal Agent of Bacterial Wilt of Leguminous Plants.- Specific Detection of Pseudomonas savastanoi pathovars: From End-Point PCR to Real-Time PCR and HRMA.- Computational Analysis of HTS Data and Its Application in Plant Pathology.- Biomonitoring of Fungal and Oomycete Plant Pathogens by Using Metabarcoding.- Phytobiome Metabarcoding: A Tool to Help Identify Prokaryotic and Eukaryotic Causal Agents of Undiagnosed Tree Diseases.- Plant-Fungal Interactions: Laser Microdissection as a Tool to Study Cell Specificity.- Somatic Embryogenesis as a Tool for Studying Grapevine-Virus Interaction.- A Quantitative Luminol-Based Assay for ROS Burst Detection in Potato Leaves in Response to Biotic Stimuli.- Genetic Analysis of Plant Pathogens Natural Populations.- Microsatellite Genotyping in the Chestnut Blight Fungus Cryphonectria parasitica.- A Multiplex PCR Approach to Determine Vegetative Incompatibility Genotypes and Mating Type in Cryphonectria parasitica.- A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents.- Spray-Induced Gene Silencing to Study Gene Function in Phytophthora.- Plant Sampling for Production of Essential Oil and Evaluation of Its Antimicrobial Activity In Vitro.