Phenotypic Screening

This volume provides a better understanding of the advancements in phenotypic readouts and improved disease models that generate novel biological insights and recapitulate clinically relevant biology. Chapters focus on phenotypic screening, the use of human cell models, microscopic approaches, assays to measure fat accumulation in C. elegans , the threat response in zebrafish, and protein-protein interactions in plant growth and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Phenotypic Screening: Methods and Protocols aims to make phenotypic screening approaches more accessible to a wide array of researchers throughout the academic and biotech communities.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Nutrient Stress Small-Molecule Screening Platform for Escherichia coli .- Validation and Development of an Escherichia coli Riboflavin Pathway Phenotypic Screen Hit as a Small-molecule Ligand of the Flavin Mononucleotide Riboswitch.- Phenotypic Screening of Small Molecules with Anti-malarial Activity for Three Different Parasitic Life Stages.- Phenotypic Screening for Inhibitors of a Mutant Thrombopoietin Receptor.- A cell-based Assay for Mitotic Spindle Orientation.- Isolation of Skeletal Muscle Stem Cells for Phenotypic Screens for Modulators of Proliferation.- Cell-based Methods to Identify Inducers of Human Pancreatic Beta-cell Proliferation.- High-content Imaging Phenotypic Screen for Neurogenesis Using Primary Neural Progenitor Cells.- Discovery of Modulators of Adipocyte Physiology Using Fully Functionalized Fragments.- The Ancient Genetic Networks of Obesity: Whole-animal Automated Screening for Conserved Fat Regulators.- Modulation of Threat Response in Larval Zebrafish.- A PLA-iRoCS Pipeline for the Localization of Protein-Protein Interactions in situ .- High-Dimensional Profiling: The Theta Comparative Cell Scoring Method.- Accessing the Open PHACTS Discovery Platform with workflow tools.- Quantitative Prioritization of Tool Compounds for Phenotypic Screening.- A Quantitative Systems Pharmacology Approach to Infer Pathways Involved in Complex Disease Phenotypes.- Phenotype-based High-content Screening using Fluorescent Chemical Bioprobes: Lipid Droplets and Glucose Uptake Quantification in Live Cells.- Developing High-throughput Electrophysiological Assays for Phenotypic Screening.- Testing Susceptibility of Patient Derived Organoid Cultures to Therapies: Pharmacotyping.- Techniques to Enable 1536-well Phenotypic Screening. <p