This detailed new edition complements the previous edition with updates and new chemistry, concepts, and applications in the study of peptide nucleic acids (PNAs). The focus is on in vivo properties, behavior, and innovative applications of PNA, while maintaining contributions on both chemistry and nucleic acid recognition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Peptide Nucleic Acids: Methods and Protocols, Fourth Edition aims to inspire discovery and pursuit of new exciting avenues in the broad area of artificial DNA.

Includes cutting-edge methods and Protocols Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Peptide Nucleic Acid-Fluorescence In Situ Hybridization (PNA-FISH) for the Detection and Spatial Location of Microorganisms.- Peptide Nucleic Acids for MicroRNAs Targeting: Updates and Future Perspectives.- Sequence-Directed Transcriptional Silencing Using Gamma Peptide Nucleic Acids.- Tetrahydrofuran Peptide Nucleic Acids.- PNA-Encoded Synthesis (PES) and DNA Display of Small Molecule Libraries.- Synthesis of Pyrrolidinyl PNA and Its Site-Specific Labeling at Internal Positions by Click Chemistry.- Reactive Quantum Dot-Based FRET Systems for Target-Catalyzed Detection of RNA.- Near Infrared In Vivo Whole Body Fluorescence Imaging of Peptide Nucleic Acid.- Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA and DNA.- Monomer Synthesis and On-Resin Chemical Functionalization of Gamma Peptide Nucleic Acid.- Linking Peptides and PNAs: Bioorthogonal Nitrile-Aminothiol Conjugation for Antimicrobial Applications.- Synthesis of Peptide Nucleic Acid-Zirconium Coordination Nanoparticles.- Mechanistic Studies of Antibacterial PNAs by RNA-Seq Analysis.- Synthesis of Hydroxamate-Type Siderophores and Their Conjugates with PNA for Evaluation of Iron Binding and Bacterial Uptake.- In Vitro Preclinical Evaluation of BPP-PNA Antisense Antibiotics: A Protocol for Efficacy, Safety, and Resistance Analysis.- Fmoc-Based Assembly of PNA Oligomers: Manual and Microwave-Assisted Automated Synthesis.- Structural and Physicochemical Optimization of Next-Generation Antisense Oligonucleotide with BROTHERS for Mitigating Off-Target Effects.- Carbohydrate Ligand-Mediated Precise Kidney Delivery of PNA.- Facile Preparation of PNA-Peptide Conjugates with a Polar Maleimido-Thioether Linkage.