The introduction of high-throughput sequencing with Next-Generation Sequencing (NGS) marked a significant advancement in the field. Over the past decade, NGS has helped to uncover molecular alterations that drive malignant behavior and provide crucial information for clinicians, directly influencing treatment options and significantly improving patient outcomes. This book focuses on the molecular alterations and signaling pathways identifiable through NGS and on the practical challenges encountered in daily diagnostic work. These include the management of scant or degraded material, the need to correlate molecular findings with morphology and ancillary techniques, and the limited availability of advanced sequencing platforms in many routine settings. Particular attention is given to tumor types in which NGS is still evolving or not yet fully standardized, allowing readers to appreciate both the opportunities and the limitations of the method.

Each chapter is structured either around the molecular alteration's characteristic of specific tumors or around broader theoretical concepts, with emphasis on decision-making algorithms and the resolution of common diagnostic problems. Next-Generation Sequencing in Solid Tumor Histopathology is designed not only for molecular pathology specialists but also for general surgical pathologists, who are increasingly required to interpret NGS reports and integrate them into daily diagnostic responsibilities, even in institutions without dedicated molecular facilities. The concluding sections address rapidly emerging technologies such as liquid biopsy, single-cell and spatial transcriptomics, and epigenomic and proteogenomic profiling, which are expected to enter routine practice in the near future and with which pathologists must become conversant.

Serves as a practical guide for histopathologists who want to incorporate NGS into their daily practice Covers the benefits and limitations of NGS and address the challenges of adopting this technology Explores how NGS can be applied across different histopathology specialties to deepen our understanding of solid tumors

Autorentext

Stefano Sioletic
Department of Pathology
San Camillo Forlanini Hospital
Rome, Italy

Luigi Coppola
Department of Pathology
Pertini and S. Eugenio Hospital
Rome, Italy

Inhalt

Part I: Principles of High-Throughput Sequencing in Histopathology.- Fundamentals of High-Throughput Sequencing Techniques .- Basics of Solid Tumor Pathology and Oncology .- Genetic Profiling in Solid Tumors: Identification of driver mutations, oncogenic fusions, and molecular biomarkers .- Part II: Applications and Innovations in High-Throughput Sequencing.- Gastrointestinal and Pancreatic Pathology .- Next-Generation Sequencing in Hepato-Biliary Carcinomas .- Applications and Innovations in High-Throughput Sequencing .- Next-Generation Sequencing in Malignant Mesenchymal Tumors .- Pediatric malignancies .- From Biopsy To Biology: Next-Generation Sequencing In Endometrial, Ovarian, And Cervical Carcinoma .- Next Generation Sequencing in Cutaneous Pathology .- Breast Pathology .- Head and Neck Pathology .- Next Generations Sequencing in Kidney, Bladder and Prostate Pathology.- Next generation sequencing in central nervous system tumors diagnostics.- Molecular pathology of the endocrine system .- NGS in Cytipathology .- Molecular Testing in Neuroendocrine Tumours .- Part III - Emerging Technologies and Innovations.- Emerging Technologies and Innovations .- Liquid Biopsy and Circulating Biomarkers .- Part IV - Future Directions and Research Opportunities.- Future Directions and Research Opportunities.