Neisseria meningitidis

Research into this potentially deadly agent of meningitis and septicaemia is a matter of urgency, and this volume details new methods and technologies that can be deployed to further our understanding of, and ability to tackle, neisseria meningitides.

Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors. Neisseria meningitidis : Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Neisseria meningitidis : Advanced Methods and Protocols can be readily used to study other pathogens and diseases and should have broad appeal to clinical and non-clinical scientists alike; however, there is still much to learn about the meningococcus and its complex nature, which in turn continues to reveal crucial details about human biology as well.

Provides advanced, cutting-edge techniques for the study of the meningococcus Builds upon the foundation of previous books in the series to supply the most current methods and protocols Includes expert tips and implementation advice to ensure successful results Includes supplementary material: sn.pub/extras

Inhalt

Neisseria meningitidis: Biology, Microbiology, and Epidemiology.- Classification and Pathogenesis of Meningococcal Infections.- Detection of Neisseria meningitidis in Cerebrospinal Fluid Using a Multiplex PCR and the Luminex Detection Technology.- Generating Knock-Out and Complementation Strains of Neisseria meningitides .- Identification and Functional Characterization of sRNAs in Neisseria meningitides .- Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins.- Genome-Scale Metabolic Models: Reconstruction and Analysis.- TMT Labeling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome.- Meningococcal Ligands and Molecular Targets of the Host.- In Vivo Imaging of Meningococcal Disease Dynamics.- Methods for Studying Neisseria meningitidis Biofilms.- A Laminar-Flow Chamber Assay for Measuring Bacterial Adhesion Under Shear Stress.- Techniques to Measure Pilus Retraction Forces.- Human Dendritic Cell Culture and Bacterial Infection.- Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry (H/DX-MS) to Investigate Ligand-Receptor Interactions.- Visualizing PAMP-PRR Interactions Using Nanoscale Imaging.- Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells.- The Use of the Pan- Neisseria Microarray and Experimental Design for Transcriptomics Studies of Neisseria.- Analysis of Parameters Associated with Prevention of Cellular Apoptosis by Pathogenic Neisseriae and Purified Porins.- Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach.- Antigen Identification Starting from the Genome: A "Reverse Vaccinology" Approach Applied to MenB.- A DNA Vaccine Strategy for Effective Antibody Induction to Pathogen-Derived Antigens.