Neisseria meningitidis

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Research into this potentially deadly agent of meningitis and septicaemia is a matter of urgency, and this volume details new meth...

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Research into this potentially deadly agent of meningitis and septicaemia is a matter of urgency, and this volume details new methods and technologies that can be deployed to further our understanding of, and ability to tackle, neisseria meningitides.
Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors. Neisseria meningitidis: Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neisseria meningitidis: Advanced Methods and Protocols can be readily used to study other pathogens and diseases and should have broad appeal to clinical and non-clinical scientists alike; however, there is still much to learn about the meningococcus and its complex nature, which in turn continues to reveal crucial details about human biology as well.
Provides advanced, cutting-edge techniques for the study of the meningococcus Builds upon the foundation of previous books in the series to supply the most current methods and protocols Includes expert tips and implementation advice to ensure successful results Includes supplementary material: sn.pub/extras
Klappentext
Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors. Neisseria meningitidis: Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neisseria meningitidis: Advanced Methods and Protocols can be readily used to study other pathogens and diseases and should have broad appeal to clinical and non-clinical scientists alike; however, there is still much to learn about the meningococcus and its complex nature, which in turn continues to reveal crucial details about human biology as well.
Inhalt
1. Neisseria meningitidis: Biology, Microbiology, and Epidemiology Nadine G. Rouphael and David S. Stephens 2. Classification and Pathogenesis of Meningococcal Infections Petter Brandtzaeg and Marcel van Deuren 3. Detection of Neisseria meningitidis in Cerebrospinal Fluid Using a Multiplex PCR and the Luminex Detection Technology Jens Kjølseth Møller 4. Generating Knock-Out and Complementation Strains of Neisseria meningitides Vincent van Dam and Martine P. Bos 5. Identification and Functional Characterization of sRNAs in Neisseria meningitides Yvonne Pannekoek and Arie van der Ende 6. Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins Muhammad Saleem, Jeremy Moore, and Jeremy P. Derrick 7. Genome-Scale Metabolic Models: Reconstruction and Analysis Gino J.E. Baart and Dirk E. Martens 8. TMT Labeling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome Karsten Kuhn, Christian Baumann, Jan Tommassen, and Thorsten Prinz 9. Meningococcal Ligands and Molecular Targets of the Host Darryl J. Hill and Mumtaz Virji 10. In Vivo Imaging of Meningococcal Disease Dynamics Hong Sjölinder and Ann-Beth Jonsson 11. Methods for Studying Neisseria meningitidis Biofilms Michael A. Apicella, Jianqiang Shao, and R. Brock Neil 12. A Laminar-Flow Chamber Assay for Measuring Bacterial Adhesion Under Shear Stress Magali Soyer and Guillaume Duménil 13. Techniques to Measure Pilus Retraction Forces Nicolas Biais, Dustin Higashi, Magdalene So, and Benoit Ladoux 14. Human Dendritic Cell Culture and Bacterial Infection Hannah E. Jones, Nigel Klein, and Garth L.J. Dixon 15. Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry (H/DX-MS) to Investigate Ligand-Receptor Interactions Jessmi M.L. Ling, Leslie Silva, David C. Schriemer, and Anthony B. Schryvers 16. Visualizing PAMP-PRR Interactions Using Nanoscale Imaging Kathy Triantafilou and Martha Triantafilou 17. Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells Biju Joseph, Matthias Frosch, Christoph Schoen, and Alexandra Schubert-Unkmeir 18. The Use of the Pan-Neisseria Microarray and Experimental Design for Transcriptomics Studies of Neisseria Nigel J. Saunders and John K. Davies 19. Analysis of Parameters Associated with Prevention of Cellular Apoptosis by Pathogenic Neisseriae and Purified Porins Paola Massari and Lee M. Wetzler 20. Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach Jeannette N. Williams, Myron Christodoulides, and John E. Heckels 21. Antigen Identification Starting from the Genome: A "Reverse Vaccinology" Approach Applied to MenB Emmanuelle Palumbo, Luigi Fiaschi, Brunella Brunelli, Sara Marchi, Silvana Savino, and Mariagrazia Pizza 22. A DNA Vaccine Strategy for Effective Antibody Induction to Pathogen-Derived Antigens Jason Rice and Myron Christodoulides