Nanoscale Imaging

This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
High Resolution Atomic Force Microscopy Imaging of Nucleic Acids.- Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing.- AFM-Based Single-Molecule Force-Spectroscopy of Proteins.- High Resolution AFM-Based Force Spectroscopy.- Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions.- Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers.- AFM-Based Characterization of Electrical Properties of Materials.- Supported Lipid Bilayers for Atomic Force Microscopy Studies.- Quantifying Small Molecule Binding Interactions with DNA Nanostructures.- Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy.- High Resolution and High-Speed Atomic Force Microscope Imaging.- High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies.- Direct Observation of Dynamic Movement of DNA Moleculesin DNA Origami Imaged using High-Speed AFM.- Assembly of Centromere Chromatin for Characterization By High-Speed Time-Lapse Atomic Force Microscopy.- High-Speed Force Spectroscopy for Single Protein Unfolding.- Probing RNA-Protein Interactions with Single-Molecule Pull-Down Assays.- Preparing Frozen-Hydrated Protein-Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging.- Probing Chromatin Structure with Magnetic Tweezers.- Single Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly.- Correlative Atomic Force and Single Molecule Fluorescence Microscopy of Nucleoprotein Complexes.- Sensing the Ultrastructure of Bacterial Surfaces and their Molecular Binding Forces using AFM.- Nanoscale Visualization of Bacterial Microcompartments using Atomic Force Microscopy.- Time-Resolved Imaging of Bacterial Surfaces using Atomic Force Microscopy.- Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levelsby AFM-Based Force Spectroscopy.- Fluorescence Correlation Spectroscopy on Genomic DNA in Living Cells.- Three-Dimensional Tracking of Quantum Dot-Conjugated Molecules in Living Cells.- AFM Indentation Analysis of Cells to Study Cell Mechanics and Pericellular Coat.- Imaging of Soft and Biological Samples using AFM Ringing Mode.- Probing Single Virus Binding Sites on Living Mammalian Cells using AFM.- Applications of Atomic Force Microscopy for Adhesion Force Measurements in Mechanotransduction.- Methods for Atomic Force Microscopy of Biological and Living Specimens.- Single Molecule Imaging in Live Embryos using Lattice Light-Sheet Microscopy.- Silver Filler Pre-Embedding to Enhance Resolution and Contrast in Multidimensional SEM: A Nanoscale Imaging Study on Liver Tissue.- Nanoscale Dynamics and Energetics of Proteins and Protein-Nucleic Acid Complexes in Classical Molecular Dynamics Simulations.