Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topic...
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Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
The proteome consists of a complex mixture of proteins each of which need to be folded correctly in order to function for the health of the organism, and many of these proteins require molecular chaperones to reach the correct conformation and, in some cases, to remain in a folded form. In Molecular Chaperones: Methods and Protocols, expert researchers address a wide variety of approaches to the study these mechanisms, featuring the workings of heat shock proteins and heat shock transcription factors, in vitro and in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters features introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Molecular Chaperones: Methods and Protocols serves as an ideal guide for all scientists who wish to pursue this vital biological action and its impact on human health and disease.
Approaches the study of heat shock transcription factors both in vivo and in vitro Features step-by-step protocols which are tested and ready for the lab Includes expert tips and vital implementation advice to ensure successful results Inhalt 1.Targeted Deletion of Hsf1, 2, and 4 Genes in Mice Xiongjie Jin, Binnur Eroglu, Demetrius Moskophidis, and Nahid F. Mivechi 2.The Role Of Heat Shock Factors in Stress-Induced Transcription Yue Zhang, Shiuh-Dih Chou, Ayesha Murshid, Thomas L. Prince, Sheila Schreiner, Mary Ann Stevenson, and Stuart K. Calderwood 3.Hsp90 and Client Protein Maturation Natalie Wayne, Parul Mishra, and Daniel N. Bolon 4.The Role of p23, Hop, Immunophilins, and Other Co-Chaperones in Regulating Hsp90 Function Marc B. Cox and Jill L. Johnson 5.Detecting Hsp90 Phosphorylation Mehdi Mollapour and Len Neckers 6. Role of Molecular Chaperones in Biogenesis of the Protein Kinome Atin K. Mandal, Maria A. Theodoraki, Nadinath B. Nillegoda, and Avrom J. Caplan 7. Nucleotide Exchange Factors for Hsp70 Chaperones Heike Rampelt, Matthias P. Mayer, and Bernd Bukau 8.Reconstitution of CHIP E3 Ubiquitin Ligase Activity Hong Yu Ren, Cam Patterson, Douglas M. Cyr, and Meredith F.N. Rosser 9.Structure-Functions of HspB1 (Hsp27) André-Patrick Arrigo 10. Combined Lentiviral and RNAi Technologies for the Delivery and Permanently Silencing of the hsp25 Gene Punit Kaur, Ganachari M. Nagaraja, Khalid Bin Abdulrahman, and Alexzander Asea 11. Quantification of HSP27 and HSP70 Molecular Chaperone Activities Jessica Gobbo, Caroline Gaucher-Di-Stasio, Stéphanie Weidmann, Jean Guzzo, and Carmen Garrido 12.Measuring Hsp72 (HSPA1A) by Indirect Sandwich ELISA H. Elyse Ireland and John H.H. Williams 13. Analysis of Heat Shock Protein Localization Using Flow Cytometry Nina C. Dempsey, Francesca Leoni, Christine Hoyle, and John. H.H. Williams 14.Quantitation of Heat Shock Proteins in Clinical Samples Using Mass Spectrometry Punit Kaur, Khalid Bin Abdulrahman, and Alexzander Asea 15.Bioinformatic Approach to Identify Chaperone Pathway Relationship from Large-Scale Interaction Networks Yunchen Gong, Zhaolei Zhang, and Walid A. Houry 16. Hsp70: Anti-Apoptotic and Tumorigenic Protein Anne-Laure Rérole, Gaëtan Jego, and Carmen Garrido 17.Determination of Cell Survival or Death Alexander E. Kabakov, Vladimir A. Kudryavtsev, and Vladimir L. Gabai 18.Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer Francesco Cappello, Everly Conway de Macario, Giovanni Zummo, and Alberto J.L. Macario 19.Preparation of a Heat Shock Proteins 70-Based Vaccine from DC-Tumor Fusion Cells Desheng Weng, Stuart K. Calderwood, and Jianlin Gong 20.Isolation of Heat Shock Protein Complexes Fernando D. Cuello-Carrión, Mariel A. Fanelli, Niubys Cayado-Gutiérrez, Gisela Castro, and Daniel R. Ciocca 21.Enhancing Antigen Cross-Presentation and T-Cell Priming by Complexing Protein Antigen to Recombinant Large Heat Shock Protein Xiang-Yang Wang, Huanfa Yi, Xiaofei Yu, Damin Zuo, and John R. Subjeck 22. Investigating Receptors for Extracellular Heat Shock Proteins Ayesha Murshid, Jimmy Theriault, Jianlin Gong, and Stuart K. Calderwood 23. Analysis of Cellular Migration Using a Two-Chamber Methodology Michelle M. Kouspou and John T. Price