This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. MicroRNA Technologies also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression profiling by PCR array in 2D and 3D neural culture systems; analysis of cortical neuron migration using miRNA silencing; isolating and screening subcellular miRNAs in neurons; and measuring miRNA mediated translational regulation with live cell imaging. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.

Cutting-edge and practical, MicroRNA Technologies is a valuable resource for students and researchers who are interested about microRNAs in nervous systems.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains keynotes and implementation advice from the experts

Inhalt

Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord.- Protocol for High Content Screening for the Impact of Overexpressed MicroRNAs on Primary Motor Neurons.- Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-Radioactive Fluorescence Method.- Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treated with the Anesthetic Protocol.- MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Target Validation.- Study of miRNA Function in the Developing Axons of Mouse Cortical Neurons: Use of Compartmentalized Microfluidic Chambers and In Utero Electroporation.- Functional Analysis of Cortical Neuron Migration Using miRNA Silencing.- Continuous Delivery of Oligonucleotides into the Brain.- Isolating and Screening Subcellular miRNAs in Neuron.- Experimental Methods for Functional Studies in MicroRNAs in Animal Models of Psychiatric Disorders.- Isolation and Quantitative Analysis of Axonal Small Noncoding RNAs.- In Ovo Electroporation of miRNA Plasmids to Silence genes in a Temporally and Spatially Controlled Manner.- Three Dimensional Cell Culture of Human Neural Stem Cells using Polysaccharide-Based Hydrogels and Subsequent Bioanalyses.- Measuring miRNA Mediated Translational Regulation with Live Cell Imaging.