MicroRNA Profiling

This volume includes comprehensive descriptions of miRNA biogenesis and their role in the development and progression of various human diseases. The first few chapters of MicroRNA Profiling: Methods and Protocols discuss the effects of over-expressing and repressing of a target miRNA and their effects on cell viability and proliferation. The next few chapters explore the protocols for total RNA isolation from cells and cell-derived product including formalin fixed paraffin embedded tissue and plant tissue. The last few chapters discuss isolation and characterization of exosomes from medium conditioned by cell lines, serum, and plasma specimens. This book also includes discussions of several software tools, such as miRandola, PicTar, DIANA, and miRWalk. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, MicroRNA Profiling: Methods and Protocols is a valuable resource for anyone interested in the field of Micro RNAs.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains keynotes and implementation advice from the experts

Inhalt

MiRNA Biogenesis and Regulation of Diseases: An Overview.- Assessment of Basic Biological Functions Exerted by miRNAs.- Extraction of miRNAs from Formalin Fixed Paraffin Embedded (FFPE) Tissues.- MiRNA Isolation from Plants Rich in Polysaccharides and Polyphenols.- MicroRNA Profiling of Exosomes.- MiRNA Profiling in Human Induced Pluripotent Stem Cells.- MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells.- TaqMan Low Density Array: MicroRNA Profiling for Biomarker and Oncosuppressor Discovery.- Detection of MicroRNAs in Brain Slices Using In Situ Hybridization.- Exosomal MicroRNA Discovery in Age-Related Macular Degeneration.- Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts.- Circulating MicroRNAs in Cancer.- Profiling Circulating MiRNAs from the Plasma of Individuals with Metabolic Syndrome.- Manipulating MiRNA Expression to Uncover Hidden Functions.- Analysis of the Distribution Profiles of Circulating MicroRNAs by Asymmetrical Flow Field Flow Fractionation.- MicroRNA Expression Profiling Using Agilent One-Color Microarray.- A Multiplex Ligation Assay for MiRNA Copy Number Profiling.- Practical Bioinformatics Analysis of MiRNA Data Using Online Tools.- Visualization and Analysis of MiRNA-Targets Interactions Networks.- Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line.- Customization of Artificial MicroRNA Design.