This volume discusses the latest analytical approaches used to sample defined molecular populations of metabolites via functional group derivatization, specialized chromatographic methods, and ionization techniques. Chapters cover key methods for sample introductions to the ion source, including direct flow, gas chromatography, liquid chromatography, and capillary electrophoresis. Chapters also explore non-targeted and targeted analyses, as well as the emerging field of metallomics. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Metabolomics is a valuable resource for students, researchers, practicing physicians and veterinarians, and administrators involved in the funding of research.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Flow Infusion ESI High Resolution Mass Spectrometry Metabolomics Analytical Platforms.- High Throughput Metabolomics using Flow Injection Analysis and Fourier Transform Ion Cyclotron Resonance-Mass Spectrometry.- Two-Dimensional Liquid Chromatography in Metabolomics and Lipidomics.- Metabolomics of Small Numbers of Cells using Chemical Isotope Labeling Combined with Nanoflow LC-MS.- Untargeted Metabolomics to Interpret the Effects of Social Defeat: Integrating Chemistry and Behavioral Approaches in Neuroscience.- Flow Infusion Electrospray Ionization High Resolution Mass Spectrometry of the Chloride Adducts of Sphingolipids, Glycerophospholipids, Glycerols, Hydroxy Fatty Acids, Fatty Acid Esters of Hydroxy Fatty Acids (FAHFA), and Modified Nucleosides.- High Mass Accuracy Gas Chromatography/Mass Spectrometry for Performing Isotopic Ratio Outlier Analysis: Applications for Non-Annotated Metabolite Detection.- Enantioseparation and Detection of (R)-2-hydroxyglutarate and (S)-2-hydroxyglutarate by Chiral Gas Chromatography-Triple Quadrupole Mass Spectrometry.- Enantioselective Supercritical Fluid Chromatography (SFC) for Chiral Metabolomics.- Capillary Electrophoresis-Mass Spectrometry of Hydrophilic Metabolomics.- Lipidomic Analysis of Oxygenated Polyunsaturated Fatty Acid-Derived-Inflammatory Mediators in Neurodegenerative Diseases.- The Characterization and Quantification of the Fatty Acid Amidome.- Metabolomics Analysis of Complex Biological Specimens using Nuclear Magnetic Resonance Spectroscopy.- Untargeted Metabolomics Methods to Analyze Blood Derived Samples.- Multi-Compartmental High-Throughput Metabolomics Based on Mass Spectrometry.- GC-MS Non-Targeted Metabolomics of Neural Tissue.- GC-MS of Pentafluourobenzyl Derivatives of Phenols, Amines, Carboxylic Acids.- GC-MS of tert-Butyldimethylsilyl (tBDMS) Derivatives of Neurochemicals.- UHPLC-MS/MS Method for Determination of Biologically Important Thiols in Plasma using New Derivatizing Maleimide Reagent.- Untargeted Metabolomics Determination of Postmortem Changes in Brain Tissue Samples by UHPLC-ESI-QTOF-MS and GC-EI-Q-MS.- Metallomics Imaging.