This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectinsthese chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Structural database for Lectins and the UniLectin Web Platform.- Purification of Sugar-Binding Peptides from L-Type Lectins.- Recombinant Expression and Purification of Animal Intracellular L-Type Lectins.- Frontal Affinity Chromatography: A Highly Suitable Retardation Phenomenon-Based research Tool for Analyzing Weak Interactions between Biomolecules.- Metazoan Soluble b-Galactoside-Binding Lectins, Galectins: Methods for Purification, Characterization of their Carbohydrate Binding Specificity, and probing their Ligands.- Expression, S-Nitrosylation, and Measurement of S-Nitrosylation Ratio of Recombinant Galectin-2.- Expression and Purification of Full-Length and Domain-Fragment Recombinant Pentraxin 3 (PTX3) Proteins from Mammalian and Bacterial Cells.- Identification of Siglec Cis-Ligands by Proximity Labeling.- Preparation of Recombinant Siglecs and Identification of their Ligands.- Purification, Quantification, and FunctionalAnalysis of Collectins.- Selectin-Binding Assay by Flow-Cytometry.- Direct Binding Analysis between C-Type Lectins and Glycans Using Immunoglobulin-Receptor Fusion Proteins.- Expression and Characterization of Hyaluronan Binding Protein Involved in Hyaluronan Depolymerization: HYBID, alias KIAA1199 and CEMIP.- Paracoccin: Purification and validation of its Lectin and Enzymatic Properties.- In Vitro Mannosidase Assay of EDEMs: ER Degradation-Enhancing b-Mannosidase-Like Proteins.- Galactose-Specific, Hemolytic Lectin CEL-III from Cucumaria echinata.- Simple and Rapid Detection of Glycoforms by Lectin Inhibition Assay.- Glycoform-Specific Visualization in Immunohistochemistry by Lectin Inhibition.- Botulinum Hemagglutinin: Critical protein for Adhesion and Absorption of Neurotoxin Complex in Host Intestine.- Functional Analysis of Botulinum Hemagglutinin (HA).- Purification and Functional Characterization of the Effects onCell Signaling of Mytilectin: A Novel -Trefoil Lectin from Marine Mussels.- Lectin-Type Ubiquitin Ligase Subunits: Fbs Proteins and their Applications for Use.- F-Type Lectins: Structure, Function, and Evolution.- Purification and Biochemical Characterization of Selected F-Type Lectins.- LecA (PA-IL): A Galactose-Binding Lectin from Pseudomonas aeruginosa.- Glycan Recognition and Application of P-Type Lectins.- Purification and Assays of Tachylectin-5.- Preparation of Soluble Malectin and its Tetramer.- Calnexin/Calreticulin and Assays Related to N-Glycoprotein Folding In Vitro.- Purification and Assays of Tachylectin-2.- Purification and Assays of Tachycitin.- Methods for Purifying Datura stramonium Agglutinin and Producing Recombinant Agglutinin Protein in a Heterologous Plant Host.- ZG16p, An Animal Homologue of Plant -Prism Fold Lectins: Purification Methods of Natural and Recombinant ZG16p and Inhibition Assay of CancerCell Growth using ZG16p.- ArtinM: Purification and Evaluation of Biological Activities.- Bacterial Expression of Rhamnose-Binding Lectin from Catfish Eggs.- A Bioassay for Determining Symbiotic Zooxanthellae Shape Control Using Lectin SLL-2 from the Octocoral Sinularia lochmodes.- MIC4 from Toxoplasma gondii: A Lectin Acting as a Toll-Like Receptor Agonist.- Production and Characterization of MIC1: A Lectin from Toxoplasma gondii.- Affinity Labeling and Purification of Plant Chitin-Binding LysM-Receptor with Chitin Octasaccharide Derivatives.- Purification of GNA-Related Lectins from Natural Sources.- Expression, Purification, and Applications of the Recombinant Lectin PVL from Psathyrella velutina Specific for Terminal N-Acetyl-Glucosamine.- Yeast Flocculin: Methods for Quantitative Analysis of Flocculation in Yeast Cells.- Pleurotus cornucoiae Mycelial Lectin (PCL-M): Purification and Detection of the Acitivty on Mycelial Surface.- Expression and Purification of a Human Pluripotent Stem Cell-Specific Lectin Probe, rBC2LCN.- Preparation of Fluorescent Recombinant Shiga Toxin-B-Subunit and its Application to Flow Cytometry.- LecB, A High Affinity Soluble Fucose-Binding Lectin from Pseudomonas aeruginosa.- Sialoglycovirology of Lectins: Sialyl Glycan Binding of Enveloped and Non-Enveloped Viruses.- Hemagglutinin Inhibitors are Potential Future Anti-Influenza Drugs for Mono- and Combination-Therapies.- Preparation and Detection of Glycan Binding Activity of Influenza Virus.- Screening for Components/Compounds with Anti-Rotavirus Activity: Detection of Interaction between Viral Spike Proteins and Glycans.- ELISA-Based Methods to Detect and Quantify Norovirus Virus-Like Particle Attachment to Histo-Blood Group Antigens.- FAM3B/PANDER-Like Carbohydrate-Binding Domain in a Glycosyltransferase, POMGNT1.- Mannose-Specific Oyster Lectin CGL1.- Receptor Binding Assays of Enterovirus D68.- Large-Scale Expression and Purification of Mumps Virus Hemagglutinin-Neuraminidase for Structural Analyses and Glycan-Binding Assays.- Purification of AJLec: A Novel Galactose-Specific Lectin, from the Sea Anemone Anthopleura japonica.- Annexin Lectins: Ca2+-Dependent Heparin-Binding Activity, Phosphatidylserine-Binding Activity, and Anticoagulant Activity.- Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans.- The OAAH Family: Anti-Influenza Virus Lectins.