Drug Metabolism and Transport

A compendium of proven experimental approaches and strategies for studying the bioactivation, detoxification, tissue distribution, and elimination of xenobiotics in the metabolism and/or transport of various chemicals. The authors address several of the major drug metabolizing systems, including the cytochrome P450 family, flavin-containing monooxygenases, glutathione, S-transferase, glucuronidation, N-acetylation, and sulfotransferases. Additional chapters present novel approaches to the study of: signaling pathways in the regulation of drug metabolism enzymes, how the modulation of thiols and other low molecular-weight cofactors can alter drug metabolism, and how modulation of drug metabolism pathways can influence antiviral therapy.

"With the diverse subject matter and the wealth of information on techniques and research strategies there is likely to be something in this book to interest those from PhD students to more experienced researchers." -Newsletter of the British Toxicological Society

Klappentext
Remarkable advances in molecular and cell biology and the development of novel in vitro model systems have greatly expanded our knowledge of the processes involved in metabolism and transport and led to numerous new therapeutic approaches. In Drug Metabolism and Transport: Molecular Methods and Mechanisms, well-established investigators describe their best experimental approaches and strategies for studying the bioactivation, detoxification, tissue distribution, and elimination of xenobiotics in the metabolism and/or transport of various chemicals. The authors address several of the major drug metabolizing systems, including the cytochrome P450 family, flavin-containing monooxygenases, glutathione, glucuronidation, N-acetylation, and sulfotransferases. Additional chapters present novel approaches to the study of: signaling pathways in the regulation of drug metabolism enzymes, how the modulation of thiols and other low-molecular-weight cofactors can alter drug metabolism, and how modulation of drug metabolism pathways can influence antiviral therapy. The strategies detailed for transport phenomena cover the multidrug resistance proteins, the reduced folate carrier, the plasma membrane, and mitochondrial glutathione transporters, and can be applied to numerous other transport systems. Many chapters contain step-by-step methods for investigating metabolism and transport.
Insightful and broad-ranging, Drug Metabolism and Transport: Molecular Methods and Mechanisms offers novice and experienced investigators alike a highly useful compendium of key strategies and approaches to successful research on drug metabolism and transport pathways.

Inhalt
Renal Cytochrome P450s and Flavin-Containing Monooxygenases.- Experimental Approaches for the Study of Cytochrome P450 Gene Regulation.- Insulin and Growth Factor Signaling.- Catalytic Function and Expression of Glutathione Transferase Zeta.- Glucuronidation of Fatty Acids and Prostaglandins by Human UDP-Glucuronosyltransferases.- Transcriptional Regulation of UDP-Glucuronosyltransferases.- Phenotypic and Genotypic Characterization of N-Acetylation.- Methods and Approaches to Study Metabolism and Toxicity of Acetaminophen.- Modulation of Drug Metabolism and Antiviral Therapies.- Modulation of Thiols and Other Low-Molecular-Weight Cofactors.- Multidrug Resistance Proteins and Hepatic Transport of Endo- and Xenobiotics.- Structural Determinants of Folate and Antifolate Membrane Transport by the Reduced Folate Carrier.- Glutathione Transport in the Kidneys.- Human Cytosolic Sulfotransferases.