Lectin Methods and Protocols brings together a comprehensive collection of optimized methods for using lectins in biomedical research. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells. Also included are step-by-step methods for the separation of human bone marrow cells for clinical use, for the assessment of the biological effects of plant lectins, and for the use of light and electron microscopy in analyzing carbohydrate structures. Taken together, the methods detailed here offer extremely useful tools for examining structural changes in glycosylation and their functional consequences for human pathophysiology

Lectin Methods and Protocols provides invaluable, easily reproducible techniques suitable for application to a wide range of important biomedical problems. The book will immediately become the standard practical resource and guide to lectin techniques for today's biomedical researchers.

Klappentext

A comprehensive collection of optimized methods for using lectins in biomedical research. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells. Also included are detailed methods for the separation of human bone marrow cells for clinical use, for the assessment of the biological effects of plant lectins, and for the use of light and electron microscopy in analyzing carbohydrate structures. Extremely useful tools for examining structural changes in glycosylation and their functional consequences for human pathophysiology.

Inhalt
Lectin Histochemistry and Cytochemistry.- Light Microscopy.- Lectin Histochemistry and CytochemistryLight Microscopy.- LectinGold Histochemistry on Paraffin and Lowicryl K4M Sections Using Biotin and Digoxigenin-Conjugated Lectins.- Use of Fluorochrome-Labeled Lectins in Light Microscopy.- The Use of Lectins in Combination with Enzymatic Digestion for the Study of Glycoconjugates in Cartilage.- Applications of Lectin Histochemistry and Cytochemistry in Diagnosis and Prognosis.- Electron Microscopy.- Electron Microscopy.- Amplification of Lectin-Gold Histochemistry.- Electron Microscopic Methods for the Demonstration of Lectin-Binding Sites in Cancer Cell Lines.- Use of Lectins for Structural Analysis of Oligosaccharide Chains.- Application of Sequential Smith Degradation to Lectin Blots.- Blot Analysis with Lectins for the Evaluation of Glycoproteins in Cultured Cells and Tissues.- Characterization of HIV gp120 Envelope Glycoprotein by Lectin Analysis.- Use of Lectins for Characterization of O-Linked Glycans of Herpes Simplex Virus Glycoproteins.- Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins.- Use of Lectin for Detection of Agalactosyl IgG.- Lectins for Detection of Altered Glycosylation of Circulating Glycoproteins.- Detection of Altered Glycosylation of ?-Fetoprotein Using Lectin-Affinity Electrophoresis.- Use of Lectin-Affinity Electrophoresis for Quantification and Characterization of Glycoforms of ?-1 Acid Glycoprotein.- ABO(H) Blood Group Expression on Circulating Glycoproteins.- Use of Lectins in Quantification of Soluble Glycoproteins.- Lectin/Antibody Sandwich ELISA for Quantification of Circulating Mucin as a Diagnostic Test for Pancreatic Cancer.- Quantification of Intestinal Mucins.- Lectins in AffinityPurification of Soluable Glycoproteins.- Purification and Characterization of Human Serum and Secretory IgA1 and IgA2 Using Jacalin.- Use of Lectins in Affinity Purification of HIV and SIV Envelope Glycoproteins.- T-Cell Receptor Purification.- Lectins in Flow Cytometry.- Use of Monomeric, Monovalent Lectin Derivatives for Flow Cytometric Analysis of Cell Surface Glycoconjugates.- Analysis of Subcellular Components by Fluorescent-Lectin Binding and Flow Cytometry.- Lectins as Tools for Cell Purification/Purging.- Lectins as Tools for the Purification of Liver Endothelial Cells.- The Use of Soybean Agglutinin (SBA) for Bone Marrow (BM) Purging and Hematopoietic Progenitor Cell Enrichment in Clinical Bone Marrow Transplantation.- Combined Lectin/Monoclonal Antibody Purging of Bone Marrow for Use in Conjunction with Autologous Bone Marrow Transplantation in the Treatment of Multiple Myeloma.- Effects of Lectins on Mammalian Cells.- Mechanisms and Assessment of Mitogenesis.- Mitogenic Effects of Lectins on Epithelial Cells.- Use of Lectins as Mitogens for Lymphocytes.- Effect of Lectins on Uptake of Polyamines.- Effects of Lectins on Cytoskeletal Organization in Mammalian Cells.- Effect of Lectins on Protein Kinase Activity.- Lectin-Induced Calcium Mobilization in Human Platelets.- Lectin-Triggered Superoxide/H2O2 and Granule Enzyme Release from Cells.- Cytotoxic Effects of Lectins.- Application of the Lectin-Dependent Cell-Mediated Cytotoxicity Assay to Bronchoalveolar Lavage Fluid and Venous Blood Samples Collected from Canine Lung Allografts.- Effects of Lectins in Organ Culture.- The Effect of Lectins on Crypt Cell Proliferation in Organ Culture.- Effects of Lectin Ingestion.- Effects of Lectin Ingestion on Animal Growth and Internal Organs.- Lectin Ingestion.- Assessment of Lectin Inactivation by Heat and Digestion.- Lectins in The Investigation of Neuronal Trafficking.- Use of Lectins as Transganglionic Neuronal Tracers in the Study of Unmyelinated Primary Sensory Neurons.- Use of Lectins in The Investigation of Pathogen-Host Interactions.- Lectin Inhibition of Bacterial Adhesion to Animal Cells.- Inhibition of HIV Infection by Lectin Binding to CD4.- Inhibition of HIV Infection by Lectin Binding to gp120.- Use of Lectins for Drug Delivery.- Absorption Enhancement by Lectin-Mediated Endo- and Transcytosis.- The Use of Lectins for Liposome Targeting in Drug Delivery.