This volume explores the latest developments and contributions to the field of 3D bioprinting, and discusses its use for quality R&D and translation. The chapters in this book are divided into two parts: Part one covers generic themes in bioprinting to introduce novice readers to the field, while also providing experts with new and helpful information. Part two discusses protocols used to prepare, characterize, and print a variety of biomaterials, cells, and tissues. These chapters also emphasize methods used for printing defined and humanized constructs suitable for human tissue modelling in research and applicable to clinical product development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, 3D Bioprinting: Methods and Protocols is a valuable resource for researchers and bioprinting laboratories/facilities interested in learning more about this rapidly evolving technology.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
History and Trends of 3D Bioprinting.- Cell Processing for 3D Bioprinting: Quality Requirements for Quality Assurance in Fundamental Research and Translation.- Computer-Aided Design and Manufacturing (CAD/CAM) for Bioprinting.- Ethics and Policy for Bioprinting.- Extrusion Based Bioprinting: Current Standards and Relevancy for Human-Sized Tissue Fabrication.- Stereolithography 3D Bioprinting.- Characterizing Bioinks for Extrusion Bioprinting: Printability and Rheology.- Laser-Assisted Bioprinting for Bone Repair.- Bioprinting Stem Cells in Hydrogel for In Situ Surgical Application: A Case for Articular Cartilage.- 3D Bioprinting Electrically Conductive Bioink with Human Neural Stem Cells for Human Neural Tissues.- 3D Coaxial Bioprinting of Vasculature.- Principles of Spheroid Preparation for Creation of 3D Cardiac Tissue Using Biomaterial-Free Bioprinting.- Bioprinting for Human Respiratory and Gastrointestinal In Vitro Models.- Bioprinting for Skin.- 3D Bioprinting and Differentiation of Primary Skeletal Muscle Progenitor Cells.- Bioprinting Strategies for Secretory Epithelial Organoids.- Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling.