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Since the pioneering discoveries of Hodgkin, Huxley, and Katz, it has been clear that specific ion conductance pathways underlie electrical act- ity. Over the ensuing 50 years, there has been ever increasing, and occasi- ally explosive, changes in the scope of efforts to understand ion channel behavior. The introduction of patch clamp technology by Erwin Neher and Bert Sakmann about 20 years ago led to the realization of the great variety of novel ion channel species, and the subsequent revolution in cl- ing has revealed an even greater diversity of the underlying molecular entities. Today, advances in the study of ion channel structure and function c- tinue at a high pace, from angstrom resolution imaging of crystallized ch- nels to their genetic manipulations in animals. In this regard, the field is a balanced one that inquires not only what ion channel entities are there, or how they operate, but also where are these molecular electronic switches? However, this balance is not particularly well presented to the general sci- tific audience or to specialists in the field. There are plenty of wonderful and useful books and monographs, as well as conferences and meetings on v- tually every aspect of ion channel structure and function. However, we are unaware that the channel localization theme has been considered in a u- fied forum.
Includes supplementary material: sn.pub/extras
Klappentext
Although there are many useful books on virtually every aspect of ion channel structure and function, work on channel localization-determining the specific sites of ion channels and their receptors on cells-has long been neglected. In Ion Channel Localization: Methods and Protocols, active researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact cell sites of such channels and receptors. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies.
Comprehensive and rich in practical detail, Ion Channel Localization: Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms.
Inhalt
Pharmacological Labeling of Ion Channels and Receptors.- Fluorescent Calcium Antagonists Tools for Imaging of L-Type Calcium Channels in Living Cells.- Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies.- GABAA Receptors.- Localization of ATP P2X Receptors.- Small Conductance Calcium-Activated Potassium Channels in Rat Brain.- Mapping N-Type Calcium Channel Distributions with H-Conotoxins.- Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development.- High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels.- Applications of Green Fluorescent Protein (GFP) Technology.- Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells.- Confocal Imaging of GFP-Labeled Ion Channels.- Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes.- Applications of Green Fluorescent Protein (GFP) Technology.- Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models.- Mutant GFP- Based FRET Analysis of K+ Channel Organization.- Delivering Ion Channels to Mammalian Cells by Membrane Fusion.- Functional Assays in Ion Channel Localization.- Detection of Neurons Expressing Calcium- Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake.- Crystallization Technique for Localizing Ion Channels in Living Cells.- Imaging of Localized Neuronal Calcium Influx.- Using Caged Compounds to Map Functional Neurotransmitter Receptors.- Atomic Force Microscopy.- Atomic Force Microscopy of Reconstituted Ion Channels.- Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy.- Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy.- Dynamic AFM of Patch Clamped Membranes.- Localizing Ion Channels with Scanning Probe Microscopes: A Perspective.