This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Overview of Immobilized Enzymes Applications in Pharmaceutical, Chemical, and Food Industry.- Parameters for the Evaluation of Immobilized Enzymes under Process Conditions.- One-Point Covalent Immobilization of Enzymes on Glyoxyl-Agarose with Minimal Physico-Chemical Modification: Immobilized Native Enzymes.- Multi-Point Covalent Immobilization of Enzymes on Glyoxyl-Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes.- Multi-Potent Covalent Immobilization of Enzymes on Supports Activated with Epoxy Groups: Stabilization of Industrial Enzymes.- Immobilization of Enzymes on Supports Activated with Glutaraldehyde: A Very Simple Immobilization Protocol.- Very Strong but Reversible Immobilization of Enzymes on Supports Coated with Ionic Polymers.- Immobilization of Lipases by Adsorption on Hydrophobic Supports: Modulation of Enzyme Properties in Biotransformations in Anhydrous Media.- Immobilization of Enzymes on Hetero-Functional Supports: Physical Adsorption Plus Additional Covalent Immobilization.- Stabilization of Multimetic Enzymes via Immobilization and Further Cross-Linking with Aldehyde-Dextran.- Immobilization of Enzymes on Magnetic Beads through Affinity Interactions.- Immobilization of Proteins on Gold Surfaces.- Immobilization of Enzymes in Protein Films.- Encapsulation of Enzymes in Porous Capsules via particle Templating.- Enzyme Immobilization in Wall-Coated Flow Microreactors.- In Situ Immobilization of Enzymes in Biomimetic Silica.- Environmentally-Friendly Enzyme Immobilization onto MOF Materials.- Co-Immobilization and Co-Localization of Multi-Enzymes Systems on Porous Materials.- Single-Particle Kinetics of Immobilized Enzymes by Harnessing the Autofluorescence of Co-Immobilized Cofactors.- Intraparticle pH Sensing within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles.- Manufacturing of Protein-Based Biomaterials Coupling Cell-Free Protein Synthesis with Protein Immobilization.- Immobilization of Enzymes as Cross-Linked Enzyme Aggregates: General Strategy to Obtain Robust Biocatalysts.- NMR OF Immobilized Enzymes.- Whole Cell Entrapment Techniques.- Immobilization of INS1E Insulin-Producing Cells within Injectable Alginate Hydrogels.- Immobilization of Cells on Polyurethane Foam.- Covalent Immobilization of Microbial Cells on Microchannel Surfaces.- Immobilization of Cells by Magnetic Nanoparticles.- Cultivation of Productive Biofilms in Flow-Reactors and their Characterization by CLSM.- Immobilization of Microalgae.- Therapeutic Applications of Encapsulated Cells.