High Content Screening

High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell. High Content Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery discusses its use as a high throughput platform to understand the functions of genes, RNA, proteins, and other cellular constituents at the level of the living cell.

Includes supplementary material: sn.pub/extras

Inhalt
to High Content Screening.- Past, Present, and Future of High Content Screening and the Field of Cellomics.- A Pharmaceutical Company User's Perspective on the Potential of High Content Screening in Drug Discovery.- Linking Microscopy and High Content Screening in Large-Scale Biomedical Research.- Instrumentation, Biological Application Software, and Sample Preparation.- Requirements, Features, and Performance of High Content Screening Platforms.- Characteristics and Value of Directed Algorithms in High Content Screening.- Characteristics and Value of Machine Learning for Imaging in High Content Screening.- Tools for Quantitative and Validated Measurements of Cells.- Automated Cell Plating and Sample Treatments for Fixed Cells in High Content Assays.- Differentiating Primary Human Cells in Rapid-Throughput Discovery Applications.- Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel.- Reagents.- Reagents to Measure and Manipulate Cell Functions.- Fluorescent Proteins and Engineered Cell Lines.- Optimizing the Integration of Immunoreagents and Fluorescent Probes for Multiplexed High Content Screening Assays.- The HaloTag™.- Protein Labeling With FlAsH and ReAsH.- Exploiting Network Biology to Improve Drug Discovery.- Physiological Indicators of Cell Function.- The Use of siRNA to Validate Immunofluorescence Studies.- Caged Substrates Applied to High Content Screening.- Informatics and Bioinformatics.- Overview of Informatics for High Content Screening.- Large-Scale Data Management for High Content Screening.- An Integrated Biomedical Knowledge Extraction and Analysis Platform.- Visualization of High Content Screening Data.- Pathway Mapping Tools for Analysis of High Content Data.- Assays and Applications of High Content Screening.- SystemsBiology in Cancer Research.- Target Validation in Drug Discovery.- High Content Screening as a Screening Tool in Drug Discovery.- Discovery of Protein Kinase Phosphatase Inhibitors.- High Content Translocation Assays for Pathway Profiling.- In Vitro Cytotoxicity Assessment.- Neurite Outgrowth in Retinal Ganglion Cell Culture.