Molecular and Cellular Enzymology

The originality of « Molecular and Cellular Enzymology" lies in the fact that it presents, not only the molecular, structural and mechanistic aspects of Enzymology, but also the cellular aspects involved in cell biology and metabolism. This book addresses not only experienced enzymologists but also students, teachers and academic and industrial scientists who are confronted with enzymological problems during their fundamental or applied research. Besides the theoretical developments, this book provides a wealth of practical information for experimentalists.

A useful and practical reference A working tool in daily laboratory practice For the first time different molecular and cellular aspects of enzymology are presented together Includes supplementary material: sn.pub/extras

Inhalt
Thermodynamics of Enzyme Reactions.- General Introduction.- The Laws of Thermodynamics Concept of Chemical Equilibrium.- Protein-Ligand Association Equilibria.- Living Beings, Open Systems.- Kinetics of Enzymes Reactions in Solution.- Chemical Kinetics.- Kinetics of Enzymatic Reactions with Michaelian Behaviour.- Experimental Methods to Study Enzymatic Reactions.- Formation and Structure of the Active Centre of Enzymes.- Enzyme Origin and Evolution.- Formation of the Functional Structure of Enzymes: Co- and Post-Translational Events.- Topology of the Active Centre of Enzymes.- The Catalytic Function.- Formation of Enzyme-Substrate Complexes.- Catalytic Mechanisms.- Examples of Structure-Function Relationships in Enzymatic Systems.- Regulation of Enzyme Activity.- Regulation by Non-Covalent Interactions.- Regulation by Covalent Modification.- Multifunctional Enzymes, Multi-Enzymatic Complexes and Metabolic Channelling.- Enzymology in Structured Environment.- Localisation and Cellular Compartmentalisation.- Kinetics of Enzymatic Reactions Catalysed by Immobilised Enzymes.- Metabolic Control Theory.