This reference provides the latest developments and emerging techniques used to study functional glycomics. Chapters included need...
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This reference provides the latest developments and emerging techniques used to study functional glycomics. Chapters included needed materials, reagants, step-by-step protocols, notes sections, common pitfalls to avoid and tips on troubleshooting.
The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.
Includes the latest developments and emerging state-of-the-art techniques to determine the glycome function Examines problem-driven strategy and methodology for Glycobiology researchers, both advanced and beginners, in academia and industry Provides integrated analysis approach of whole sugar chains Presents a practical manual to identify and monitor modifications and changes in glycomes for biomarker discovery and vaccines Includes supplementary material: sn.pub/extras Inhalt 1. Functional Glycomics and Glycobiology: An Overview Jianjun Li and James C. Richards 2. Historical Overview of Glycoanalysis Alicia M. Bielik and Joseph Zaia 3. Quantitative Glycomics Ron Orlando 4. Human Gangliosides and Bacterial Lipo-oligosaccharides in the Development of Autoimmune Neuropathies Nobuhiro Yuki 5. Biotinylated Multivalent Glycoconjugates for Surface Coating Alexander Chinarev, Oxana Galanina, and Nicolai Bovin 6. Profiling LPS Glycoforms of Non-typeable Haemophilus influenzae by Multiple-Stage Tandem Mass Spectrometry Elke K.H. Schweda and James C. Richards 7. In vitro Reconstitution of Escherichia coli O86 O Antigen Repeating Unit Weiqing Han, Lei Li, Nicholas Pettit, Wen Yi, Robert Woodward, Xianwei Liu, Wanyi Guan, Veer Bhatt, Jing Katherine Song, and Peng George Wang 8. Glycoprotein Characterization Susan M. Twine, Luc Tessier, and John F. Kelly 9. N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Performance Liquid Chromatography and Electrospray Tandem Mass Spectrometry S. O. Siu, Maggie P. Y. Lam, Edward Lau, William S.B. Yeung, David M. Cox, and Ivan K. Chu 10. Microarray-Based Study of Carbohydrate-Protein Binding Zhenxin Wang and Jingqing Gao 11. The Application of NMR Spectroscopy to Functional Glycomics Jean-Robert Brisson, Evgeny Vinogradov, David McNally, Nam Huan Khieu, Ian Schoenhofen, Susan Logan, and Harold Jarrell 12. Metabolomics in Glycomics Evelyn C. Soo and Joseph P. M. Hui 13. Characterization of Lipid-Linked Oligosaccharides by Mass Spectrometry Christopher W. Reid, Jacek Stupak, and Christine M. Szymanski 14. A Glycomics Approach to the Discovery of Potential Cancer Biomarkers Hyun Joo An and Carlito B. Lebrilla 15. Extraction of Chondroitin/Dermatan Sulfate Glycosaminoglycans from Connective Tissue for Mass Spectrometric Analysis Alicia M. Bielik and Joseph Zaia 16. N-Linked Protein Glycosylation in a Bacterial System Harald Nothaft, Xin Liu, David J. McNally, and Christine M. Szymanski 17. Characterization of Polysaccharides Using Mass Spectrometry for Bacterial Serotyping Eleonora Altman and Jianjun Li 18. Is Permethylation Strategy Always Applicable to Protein N-Glycosylation Study?: A Case Study on the O-Acetylation of Sialic Acid in Fish Serum Glycans Xin Liu and Luis Afonso 19. Bioinformatics in Glycomics: Glycan Characterization with Mass Spectrometric Data Using SimGlycan™ Arun Apte and Ningombam Sanjib Meitei