Clear and concise, this easy-to-use text offers an introductory course on the language of gene cloning, covering microbial, plant, and animal systems. The essential concepts in biology relevant to the understanding of gene cloning are presented in a well-organized and accessible manner. This updated version of the first edition is an invaluable book for nonscientists as well as scientists with little background knowledge in gene cloning, providing a wealth of information for anyone wishing to gain proficiency in reading and speaking the language of gene cloning.

Assumes no prior knowledge on the part of the reader Easy to understand, concise writing style teaches novices to read and speak the "language" of gene cloning Includes sample applications of gene cloning in food science, nutrition, and biomedicine, including gene therapy and cloning End-of-chapter exercises and reading assignments facilitate self-study New to this edition are extensive updates on the current state of whole genome sequencing as well as personalized medicine and gene therapy, as well as new methods of gene editing and methodologies for locating disease genes

Autorentext

Dominic W. S. Wong, Ph.D. is a scientist at the Western Regional Research Center, Albany, California, USA. Dr. Wong is also the sole author of Mechanism and Theory in Food Chemistry (1989, 2018), Food Enzymes: Structure and Mechanism (1995), and co-editor of Handbook of Food Enzymology (2003).

Inhalt
Fundamentals of Genetic Processes.- Introductory Concepts.- Structures of Nucleic Acids.- Structures of Proteins.- The Genetic Process.- Organization of Genes.- Reading The Nucleotide Sequence of a Gene.- Techniques and Strategies of Gene Cloning.- Enzymes Used in Cloning.- Techniques Used in Cloning.- Cloning Vectors for Introducing Genes into Host Cells.- Transformation.- Isolating Genes for Cloning.- Impact of Gene Cloning.- Improving Tomato Quality by Antisense Rna.- Transgenic Crops Engineered with Insecticidal Activity.- Transgenic Crops Conferred with Herbicide Resistance.- Growth Enhancement in Transgenic Fish.- Impact of Gene Cloning.- Microbial Production of Recombinant Human Insulin.- Finding Disease-Causing Genes.- Human Gene Therapy.- Gene Targeting.- DNA Typing.- Transpharmers - Bioreactors for Pharmaceutical Products.- Animal Cloning.- Human Genome Sequencing.- Manipulating Gene Vector Constructs. <p