Dendritic Cell Protocols

The third edition of this volume is aimed at providing both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells(DC). An introductory review provides an overview of recent advances in the characterization of DC subsets in mouse and human. While additional chapters provide methods to culture human and mouse dendritic cells, protocols for the isolation of dendritic cells, the isolation of dendritic cell progenitors from mouse, and the purification of dendritic cells from human blood. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Dendritic Cell Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Review of Mouse and Human Dendritic Cell Subsets.- In Vitro Generation of Human Xcr1 + Dendritic Cells from Cd34 + Hematopoietic Progenitors.- Derivation and Utilization Of Functional Cd8 + Dendritic Cell Lines.- Isolation of Dendritic Cell Progenitor and Bone Marrow Progenitor Cells from Mouse.- The Isolation and Enrichment of Large Numbers of Highly Purified Mouse Spleen Dendritic Cell Populations and their In Vitro Equivalents.- Dendritic Cell Subsets Purification from Human Tonsils and Lymph Nodes.- Isolation and Identification of Conventional Dendritic Cell Subsets from the Intestine of Mice and Men.- Isolation of Human Skin Dendritic Cell Subsets.- Isolation of Mouse Dendritic Cell Subsets and Macrophages from Skin.- Isolation of Conventional Dendritic Cells from Mouse Lungs.- Purification of Human Dendritic Cell Subsets from Peripheral Blood.- Protocols for the Identification andIsolation of Antigen-Presenting Cells in Human and Mouse Tissues.- Measurement of Export to the Cytosol in Dendritic Cells Using a Cytofluorimetry-Based Assay.- Cross-Presentation Assay for Human Dendritic Cells.- Analysis of Intracellular Trafficking of Dendritic Cell Receptors for Antigen Targeting.- Characterization of Dendritic Cell Subsets through Gene Expression Analysis.- In Vivo Ablation of a Dendritic Cell Subset Expressing a Chemokine Receptor, Xcr1.- In Vivo Analysis Of Intestinal Mononuclear Phagocytes.- In Vivo Imaging Of Cutaneous Dcs In Mice.- Analysis of Dendritic Cell Function by Clec9a-Dtr Transgenic Mice.- Analysis of Dc Functions Using Cd205-Dtr Knock-In Mice.- Generation of Humanized Mice for Analysis of Human Dendritic Cells.