Cellulases

This volume discusses the latest research and techniques used to study novel cellulases. The chapters in this book explore topics such as the role of cellulases in lignocellulose deconstruction for generation of a sugar platform for biofuel; screening for novel fungal b-glucosidases in a variety of fungal strains; discovery of novel enzymes in bacteria or fungi; protocols for isolating cellulolytic rumen bacteria; analysis of enzyme mixtures produced by ligocellulolytic fungi; and homology modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cellulases: Methods and Protocols is a valuable resource for biochemists, molecular biologists, chemical engineers, and industrial researchers interested in this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Cellulases: Role in Lignocellulosic Biomass Utilization.- Discovery of a Novel Fungus with an Extraordinary b-Glucosidase.- Discovery of New Cellulases Using Classical or Molecular Methods.- Isolation and Screening of Cellulolytic Filamentous Fungi.- Isolation and Screening of Cellulose Degrading Microorganisms from Different Ecological Niches.- Isolation of Cellulolytic Bacteria from the Rumen.- Methods for Discovery of Novel Cellulosomal Cellulases using Genomics and Biochemical Tools.- Metatranscriptomic Techniques for Identifying Cellulases in Termites and Their Symbionts.- Discovery of Novel Cellulases Using Proteomic Strategies.- Identification of Key Components for the Optimization of Cellulase Mixtures Using a Proteomic Strategy.- Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger.- Advanced Cloning Tools for Construction of Designer Cellulosomes.- Evaluation of Thermal Stability of Cellulosomal Hydrolases and Their complex Formation.- Endoglucanase (EG) Activity Assays.- Cellobiohydolase (CBH) Activity Assays.- Two-Dimensional High-Throughput Endo-Enzyme Screening Assays Based on Chromogenic Polysaccharide Hydrogel and Complex Biomass Substrates.- Analytical Tools for Characterizing Cellulose-Active Lytic Polysaccharide Monooxygenases (LPMOs).- Light Induced Electron Transfer Protocol for Enzymatic Oxidation of Polysaccharide.- Purification of Cellulase from Cellulolytic Microorganisms and Characterization of the Total Cellulase Activities (TCA).- Fungal On-Site Production of Cellulolytic Enzymes by the Sequential Cultivation Method.- Test of Efficacy of Cellulases for Biomass Degradation.- Homology Modeling for Enzyme Design.